| Autor: |
Zhang XT; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.; Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing 210095, China., Luan XP; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China., Wei JH; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China., Zhang PP; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China., Guo JM; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China., Keesey IW; School of Biological Sciences, University of Nebraska-Lincoln (UNL), Lincoln, Nebraska 68588, United States., Gao Y; College of Plant Protection, Jilin Agricultural University, Changchun 130062, China., Yan Q; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.; Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing 210095, China., Zhang J; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.; Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing 210095, China.; Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing 210095, China., Dong SL; Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.; Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing 210095, China. |
| Abstrakt: |
The bean bug Riptortus pedestris is a major soybean pest and a cause of the stay-green symptoms. However, the molecular mechanisms underlying its olfaction-mediated host-seeking behavior remain unclear. In this study, we compared the antennae transcriptomes of starved and nonstarved adult R. pedestris , identifying four differentially expressed odorant receptor (OR) genes. Among these, RpedOR13 showed a strong response to the host volatile 2-phenylethanol (2-PE) in Xenopus oocyte assays, while electroantennography and behavioral tests confirmed 2-PE as an effective attractant. Next, phylogenetic analysis identified RpedOR72b as a paralog of RpedOR13, with subsequent Xenopus oocyte assays confirming its specific response to 2-PE. Additionally, RNA interference experiments highlighted the crucial role of RpedOR72b in detecting 2-PE. Taken together, these findings provide new insights into the molecular mechanisms of host-seeking behavior in R. pedestris and highlight the successful application of reverse chemical ecology in OR-based screening of bioactive compounds. |
