Structures of Trichomonas vaginalis macrophage migratory inhibitory factor.

Autor: Srivastava A; California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA., Nair A; Reedy High School, 3003 Stonebrook Parkway, Frisco, Texas, USA., Dawson OCO; Grafton High School, 403 Grafton Drive, Yorktown, Virginia, USA., Gao R; Grafton High School, 403 Grafton Drive, Yorktown, Virginia, USA., Liu L; Protein Structure and X-ray Crystallography Laboratory, 2034 Becker Drive, Lawrence, KS 66047, USA., Craig JK; Center for Emerging and Re-emerging Infectious Diseases (CERID), Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Battaile KP; NYX, New York Structural Biology Center, Upton, New York, USA., Harmon EK; Center for Emerging and Re-emerging Infectious Diseases (CERID), Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Barrett LK; Center for Emerging and Re-emerging Infectious Diseases (CERID), Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Van Voorhis WC; Center for Emerging and Re-emerging Infectious Diseases (CERID), Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Subramanian S; Seattle Structural Genomics Center for Infectious Diseases, Seattle, Washington, USA., Myler PJ; Seattle Structural Genomics Center for Infectious Diseases, Seattle, Washington, USA., Lovell S; Protein Structure and X-ray Crystallography Laboratory, 2034 Becker Drive, Lawrence, KS 66047, USA., Asojo OA; Dartmouth Cancer Center, One Medical Center Drive, Lebanon, NH 03756, USA., Darwiche R; Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland.
Jazyk: angličtina
Zdroj: Acta crystallographica. Section F, Structural biology communications [Acta Crystallogr F Struct Biol Commun] 2024 Dec 01. Date of Electronic Publication: 2024 Dec 01.
DOI: 10.1107/S2053230X24011105
Abstrakt: The unicellular parasitic protozoan Trichomonas vaginalis causes trichomoniasis, the most prevalent nonviral sexually transmitted disease globally. T. vaginalis evades host immune responses by producing homologs of host proteins, including cytokines such as macrophage migration inhibitory factor. T. vaginalis macrophage migration inhibitory factor (TvMIF) helps to facilitate the survival of T. vaginalis during nutritional stress conditions, increases prostate cell proliferation and invasiveness, and induces inflammation-related cellular pathways, thus mimicking the ability of human MIF to increase inflammation and cell proliferation. The production, crystallization and three structures of N-terminally hexahistidine-tagged TvMIF reveal a prototypical MIF trimer with a topology similar to that of human homologs (hMIF-1 and hMIF-2). The N-terminal tag obscures the expected pyruvate-binding site. The similarity of TvMIF to its human homologs can be exploited for structure-based drug discovery.
(open access.)
Databáze: MEDLINE
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