Comparative Analysis of Gene Expression at Sea Level and High Altitude: A Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Approach.

Autor:	Alharthi SB; Molecular Diagnostic Unit, Al Hada Armed Forces Hospital, Taif, SAU.; Biological Sciences, King Abdulaziz University, Jeddah, SAU., Alsubai AH; Science Department, Shorouq Al Mamlakah International School, Taif, SAU., Almalki SK; Science Department, Shorouq Al Mamlakah International School, Taif, SAU., El-Shehawi AM; Department of Biotechnology, Taif University, Taif, SAU., Eldebsy AM; Science Department, Shorouq Al Mamlakah International School, Taif, SAU., Alsoliman AA; Molecular Diagnostic Unit, Al Hada Armed Forces Hospital, Taif, SAU., Alharthi RF; Molecular Diagnostic Unit, Al Hada Armed Forces Hospital, Taif, SAU., Morsi M; Biological Sciences, King Abdulaziz University, Jeddah, SAU., Alharthi D; Molecular Diagnostic Unit, Al Hada Armed Forces Hospital, Taif, SAU., Mutabaqani RA; Molecular Diagnostic Unit, Al Hada Armed Forces Hospital, Taif, SAU.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2024 Oct 27; Vol. 16 (10), pp. e72489. Date of Electronic Publication: 2024 Oct 27 (Print Publication: 2024).
DOI:	10.7759/cureus.72489
Abstrakt:	This research studies the gene expression in response to different oxygen environments and looks at high vs low oxygen environments. Tracking down the activity of some of these genes, namely VHLEL, VEGF and HIF-1α, quantitative real-time polymerase chain reaction (PCR) analysis was done for the study group at sea level in Jeddah and at high altitude in Taif city. It has been found that these genes are much more active in higher altitudes which indicates that there is a biological mechanism that makes those specific sites more oversized for the issue of low oxygen. This knowledge is beneficial as it helps in understanding how people grow to live in high-altitude regions. This is positive, especially in justifying the use of the methods in treatment of altitude sickness and other related diseases. Although these findings bring some hope, it would be equally important to include more participants in future studies in order to consolidate our findings and gain deeper understanding of physiological adaptation in low oxygen. This research work has potential significant contribution to the medical profession under conditions of similar environment. Competing Interests: Human subjects: Consent for treatment and open access publication was obtained or waived by all participants in this study. King Fahd Armed Forces Hospital issued approval REC 504. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Alharthi et al.)
Databáze:	MEDLINE
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