| Autor: |
Duan J; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Zhang A; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Fu Y; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Lin Y; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Xie J; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Cheng J; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Chen T; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Li B; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Yu X; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Lyu X; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Jiang D; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. |
| Abstrakt: |
Strain IBc-114 was isolated from a gray mold lesion and was identified as the fungus Schizophyllum commune. In this strain, two mycoviruses, Schizophyllum commune RNA virus 1 (ScRV1, C_AA053475.1) and Botrytis cinerea mitovirus 9 strain IBc-114 (BcMV9/IBc-114, C_AA053476.1), were isolated and characterized. ScRV1 has flexuous filamentous particles about 20 ± 2.1 nm in diameter and 1000 ± 94.2 nm in length. The genome of ScRV1 is 7370 nt in length and contains two open reading frames (ORFs) which encode a polyprotein and a coat protein, respectively. The polyprotein has 1967 aa, including a helicase domain and an RdRp domain which has the highest identity of 28.21% with that of Entomophthora benyvirus E (EbVE). The coat protein has 241 aa which is mostly phylogenetically close to the coat proteins of Alphatetraviridae . Based on the phylogenetic analysis of ScRV1 and viruses selected, ScRV1 might represent a new family (temporarily named Mycobenyviridae) of the order Hepelivirales . The genome of BcMV9/IBc-114 that infects S. commune is 2729 nt in length and has only one ORF encoding an RdRp protein with 719 aa. BcMV9/IBc-114 has the highest identity of 98.61% with Botrytis cinerea mitovirus 9 (BcMV9) (MT089704). ScRV1, but not BcMV9/IBc-114, has certain effects on the host growth of S. commune . Furthermore, BcMV9/IBc-114 has been demonstrated to replicate in the ascomycetous fungi Botrytis cinerea and Sclerotinia sclerotiorum , and it negatively affects the growth and pathogenicity of B. cinerea , but it does not affect S. sclerotiorum . This is the first report of mycoviruses in S. commune and cross-phyla transmission of mitovirus in nature. |
