Genetic Spatio-Temporal Analysis of Hepatitis D Virus Infection in Western Brazilian Amazon.

Autor: Roca TP; Laboratório de Hepatites Virais, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, RJ, Brazil.; Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia, Porto Velho 76812-245, RO, Brazil., Queiroz JAS; Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia, Porto Velho 76812-245, RO, Brazil.; Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia-UNIR, Porto Velho 76801-059, RO, Brazil., Passos-Silva AM; Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia, Porto Velho 76812-245, RO, Brazil.; Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia-UNIR, Porto Velho 76801-059, RO, Brazil., Araújo A; Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia, Porto Velho 76812-245, RO, Brazil.; Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia-UNIR, Porto Velho 76801-059, RO, Brazil., Lago BV; Laboratório de Hepatites Virais, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, RJ, Brazil.; Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, RJ, Brazil., Mello FCA; Laboratório de Hepatites Virais, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, RJ, Brazil., Salcedo JMV; Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia, Porto Velho 76812-245, RO, Brazil.; Ambulatório de Hepatites Virais, Centro de Pesquisa em Medicina Tropical-CEPEM, Porto Velho 76812-329, RO, Brazil., Vieira D; Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia, Porto Velho 76812-245, RO, Brazil., Villar LM; Laboratório de Hepatites Virais, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, RJ, Brazil.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2024 Oct 29; Vol. 16 (11). Date of Electronic Publication: 2024 Oct 29.
DOI: 10.3390/v16111690
Abstrakt: The Brazilian Amazon region is a highly endemic area for hepatitis Delta and has areas that are difficult to access. Understanding the dynamics of HDV transmission in these remote locations is important for elucidating the routes of infection. To investigate this, a molecular analysis of HDV was conducted to assess the spatio-temporal dynamics of HDV cases. Between 2010 and 2023, 35 patients were recruited from the Viral Hepatitis Outpatient Clinic in Rondônia, Brazil. Conventional PCR was used to amplify the complete HDV genome followed by nucleotide sequencing via the Sanger method. The HDV genotype was determined using maximum likelihood phylogenetic reconstruction. A Skygrid coalescent approach with a Relaxed Random Walk phylogeographic model was used for the spatio-temporal analysis. Most individuals were males (21/35), with a median age of 39 years. HDV-3 was identified in all samples (35/35; 100%). The tMRCA was estimated to be 1824, with a substitution rate of 8.2 × 10 -4 substitutions/site/year. The results suggest that HDV likely entered Brazil around 1820, in the state of Amazonas, subsequently spreading to Acre and Rondônia. Notable migration events were observed starting from 2010. This study suggests that HDV-3 has a complex evolutionary history spanning over two centuries, with intricate transmission routes in different locations in Brazil.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje