| Autor: |
Tapia-Cornejo AS; Departamento de Medicina Interna, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, Mexico., Ramírez-Castillo FY; Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico., Guerrero-Barrera AL; Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico., Guillen-Padilla DE; Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico., Arreola-Guerra JM; Departamento de Nefrología, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, Mexico., González-Gámez M; Departamento de Infectología, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, Mexico., Avelar-González FJ; Laboratorio de Estudios Ambientales, Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico., Loera-Muro A; CONAHCYT, Centro de Investigaciones Biológicas del Noreste (CIBNOR), La Paz 23205, Mexico., Hernández-Cuellar E; Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico., Ramos-Medellín CL; Laboratorio Clínico, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, Mexico., Adame-Álvarez C; Laboratorio Clínico, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, Mexico., García-Romo R; Laboratorio Clínico, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, Mexico., Galindo-Guerrero F; Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico., Moreno-Flores AC; Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico. |
| Abstrakt: |
Pseudomonas aeruginosa is a leading cause of healthcare-associated infections, which are related to substantial morbidity and mortality. The incidence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants has been previously reported in this bacterium. However, there is limited information regarding the presence of PMQR and carbapenemase-encoding genes simultaneously. This study aims to analyze the prevalence of these determinants on P. aeruginosa strain isolated from clinical patients in the State of Aguascalientes, Mexico. Fifty-two P. aeruginosa isolates from nosocomial patients were collected from Centenario Hospital Miguel Hidalgo. This is a retrospective observational study conducted at a single center. Antibiotic susceptibility was tested using the Vitek-2 system. Only carbapenem-resistant isolates were included in this study. Carbapenemase-encoding genes and PMQR determinants were screened by polymerase chain reaction (PCR). Resistance rates of 100% were found on tigecycline and ceftriaxone. Of the 52 isolates, 34.6% were positive for the qnr genes, 46.2% for the oqxA gene, and 25% for the aac-(6')-lb gene. The most frequent carbapenemase genes found in the samples were bla OXA-51 (42.3%), bla OXA-1 (15.4%), and bla VIM (15.4%). bla OXA-51 co-carrying oqxA was detected in 21.1% of the isolates, bla OXA-51 co-carrying aac-(6')-lb in 11.5%, bla VIM co-carrying aac-(6')-lb in 3.8%, and bla KPC co-carrying oqxA in 5.8%. Systematic surveillance to detect carbapenemase-encoding genes and PMQR determinants, and rational prescription using the last-line drugs could help in preventing the dissemination of multidrug-resistant determinants. |
