Emergence of Carbapenem-Resistant Uropathogenic Escherichia coli (ST405 and ST167) Strains Carrying bla CTX-M-15 , bla NDM-5 and Diverse Virulence Factors in Hospitalized Patients.

Autor: Mujahid F; Institute of Microbiology, Government College University Faisalabad, Faisalabad 38000, Pakistan., Rasool MH; Institute of Microbiology, Government College University Faisalabad, Faisalabad 38000, Pakistan., Shafiq M; Research Institute of Clinical Pharmacy, Department of Pharmacology, Shantou University Medical College, Shantou 515041, China., Aslam B; Institute of Microbiology, Government College University Faisalabad, Faisalabad 38000, Pakistan., Khurshid M; Institute of Microbiology, Government College University Faisalabad, Faisalabad 38000, Pakistan.
Jazyk: angličtina
Zdroj: Pathogens (Basel, Switzerland) [Pathogens] 2024 Nov 05; Vol. 13 (11). Date of Electronic Publication: 2024 Nov 05.
DOI: 10.3390/pathogens13110964
Abstrakt: Background: Urinary tract infections (UTIs) are common infectious diseases in hospital settings, and they are frequently caused by uropathogenic Escherichia coli (UPEC). The emergence of carbapenem-resistant (Carb-R) E. coli strains poses a significant threat due to their multidrug resistance and virulence. This study aims to characterize the antimicrobial resistance and virulence profiles of Carb-R UPEC strains isolated from hospitalized patients.
Methods: A total of 1100 urine samples were collected from patients in Lahore and Faisalabad, Pakistan, between May 2023 and April 2024. The samples were processed to isolate and identify E. coli using standard microbiological techniques and VITEK®2, followed by amplification of the uid A gene. Antimicrobial susceptibility was evaluated using the Kirby-Bauer disc diffusion method and broth microdilution. Resistance and virulence genes were detected through PCR and DNA sequencing, and sequence typing was performed using MLST.
Results: Among the 118 Carb-R UPEC isolates, resistance was most frequently observed against sulfamethoxazole-trimethoprim (96.6%) and doxycycline (96.6%). All of the isolates remained sensitive to colistin and tigecycline. Sequence types ST405 (35.6%) and ST167 (21.2%) were predominant and carried the bla CTX-M-15 and bla NDM-5 genes. The distribution of virulence genes and a variety of antimicrobial resistance genes (ARGs), conferring resistance to aminoglycosides, fluoroquinolones, tetracyclines, and sulfonamides, were observed as specifically linked to certain sequence types.
Conclusions: This study provides insights into the molecular epidemiology of carbapenem-resistant Uropathogenic E. coli (Carb-R UPEC) strains and highlights the presence of globally high-risk E. coli clones exhibiting extensive drug resistance phenotypes in Pakistani hospitals. The findings underscore the urgent need for enhanced surveillance and stringent antibiotic stewardship to manage the spread of these highly resistant and virulent strains within hospital settings.
Databáze: MEDLINE
Externí odkaz: