| Autor: |
Sallehuddin N; Department of Tissue Engineering and Regenerative Medicine, Faculty of Medicine, University Kebangsaan Malaysia, Jalan Yaacob Latif, Cheras, Kuala Lumpur 56000, Malaysia., Hao LQ; My Cytohealth Sdn. Bhd., Hive 5, Taman Teknologi, MRANTI, Bukit Jalil, Kuala Lumpur 57000, Malaysia., Wen APY; Department of Surgery, Hospital Canselor Tuanku Muhriz, Jalan Yaacob Latif, Cheras, Kuala Lumpur 56000, Malaysia., Fadilah NIM; Department of Tissue Engineering and Regenerative Medicine, Faculty of Medicine, University Kebangsaan Malaysia, Jalan Yaacob Latif, Cheras, Kuala Lumpur 56000, Malaysia.; Advance Bioactive Materials-Cells UKM Research Group, University Kebangsaan Malaysia, Bangi 43600, Malaysia., Maarof M; Department of Tissue Engineering and Regenerative Medicine, Faculty of Medicine, University Kebangsaan Malaysia, Jalan Yaacob Latif, Cheras, Kuala Lumpur 56000, Malaysia.; Advance Bioactive Materials-Cells UKM Research Group, University Kebangsaan Malaysia, Bangi 43600, Malaysia., Fauzi MB; Department of Tissue Engineering and Regenerative Medicine, Faculty of Medicine, University Kebangsaan Malaysia, Jalan Yaacob Latif, Cheras, Kuala Lumpur 56000, Malaysia.; Advance Bioactive Materials-Cells UKM Research Group, University Kebangsaan Malaysia, Bangi 43600, Malaysia. |
| Abstrakt: |
Wound infection is the leading cause of delayed wound healing. Despite ongoing research, the ideal treatment for full-thickness skin wounds is yet to be achieved. Skin tissue engineering provides an alternative treatment, with the potential for skin regeneration. Background/Objectives : Previously, we characterized a collagen-gelatin-elastin (CollaGee) acellular skin substitute and evaluated its cytocompatibility. The assessments revealed good physicochemical properties and cytocompatibility with human dermal fibroblasts (HDF). This study aimed to incorporate thymoquinone (TQ) as the antibacterial agent into CollaGee biomatrices and evaluate their cytocompatibility in vitro. Methods : Briefly, dose-response and antibacterial studies were conducted to confirm the antimicrobial activity and identify the suitable concentration for incorporation; 0.05 and 0.1 mg/mL concentrations were selected. Then, the cytocompatibility was evaluated quantitatively and qualitatively. Results : Cytocompatibility analysis revealed no toxicity towards HDFs, with 81.5 + 0.7% cell attachment and 99.27 + 1.6% cell viability. Specifically, the 0.05 mg/mL TQ concentration presented better viability, but the differences were not significant. Immunocytochemistry staining revealed the presence of collagen I, vinculin, and alpha smooth muscle actin within the three-dimensional biomatrices. Conclusions : These results suggest that TQ-incorporated CollaGee biomatrices are a promising candidate for enhancing the main key player, HDF, to efficiently regenerate the dermal layer in full-thickness skin wound healing. Further investigations are needed for future efficiency studies in animal models. |
