| Autor: |
Carbonero ER; Instituto de Química, Universidade Federal de Catalão, Catalan 75704-020, GO, Brazil., Novikov TSM; Instituto de Química, Universidade Federal de Catalão, Catalan 75704-020, GO, Brazil., Gomes YGS; Instituto de Química, Universidade Federal de Catalão, Catalan 75704-020, GO, Brazil., Brito DR; Instituto de Química, Universidade Federal de Catalão, Catalan 75704-020, GO, Brazil., Coelho LC; Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasilia 70910-900, DF, Brazil., Mendes MF; Instituto de Química, Universidade Federal de Catalão, Catalan 75704-020, GO, Brazil., Di Medeiros Leal MCB; Departamento de Medicina, Centro Universitário do Planalto Central Apparecido dos Santos, Brasilia 72445-020, DF, Brazil., Bocca AL; Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasilia 70910-900, DF, Brazil.; Plataforma Bi-Institucional de Pesquisa Translacional, Fundação Oswaldo Cruz, Ribeirao Preto 14040-900, SP, Brazil., Lião LM; Instituto de Química, Universidade Federal de Goiás, Goiânia 74690-900, GO, Brazil. |
| Abstrakt: |
Background: The consumption of nutraceuticals or food supplements has increased crucially, aiming to address nutrient deficits and enhance immune system function. To develop safe food products with unique nutritional and functional benefits, new production methods of these nutraceuticals such as the fermentative process have been gaining prominence for industrial applications. Bionutri-AR1 ® is a nutraceutical produced via this bioprocess, featuring a complex composition, that has been used to improve the immune systems of debilitated people. Objectives: Considering the various biological properties attributed to glucans, one of its main components, this study aims to structurally characterize and evaluate, in vitro, the antioxidant and immunomodulatory potential of the polymers from this nutraceutical to assess whether these polymers contribute to the product's reported biological effects. Methods/Results: Unlike previous reports, this study characterized by NMR, GC-MS, and Congo Red assay techniques two main glucans: a water-insoluble linear α-D-glucan with glycosidic bonds (1→4) and a soluble branched (1→3)- and (1→6)-linked β-glucan with a triple helix. Both glucans showed significant antioxidant activity, measured by their capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. They were also capable of inducing the secretion of cytokines such as tumoral necrosis factor-alpha (TNF-α), interleukin 10 (IL-10), and interleukin 6 (IL-6), determined through capture enzyme-linked immunosorbent assay (ELISA), especially when co-stimulated with lipopolysaccharide (LPS). Conclusions: This suggests a dual action of these glucans in both proinflammatory and regulatory pathways. Future studies will describe the mechanisms by which these glucans, especially the insoluble ones, enhance immune system function, highlighting their potential use in immunotherapy. |
