Molecular Characterization of Multidrug-Resistant and Hypervirulent New Delhi Metallo-Beta-Lactamase Klebsiella pneumoniae in Lazio, Italy: A Five-Year Retrospective Study.
| Autor: | Rotondo C; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Venditti C; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Butera O; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Dimartino V; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Messina F; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Properzi M; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Caparrelli C; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Antonelli V; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., D'Arezzo S; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Selleri M; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Nisii C; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., Fontana C; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy., On Behalf Of The Lazio Region Laboratory Study Group; National Institute for Infectious Diseases 'L. Spallanzani' Istituto di Ricovero e Cura a Carattere Scientifico, via Portuense 292, 00149 Rome, Italy. |
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| Jazyk: | angličtina |
| Zdroj: | Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2024 Nov 05; Vol. 13 (11). Date of Electronic Publication: 2024 Nov 05. |
| DOI: | 10.3390/antibiotics13111045 |
| Abstrakt: | Background/objectives: Antimicrobial resistance represents a challenge to public health systems because of the array of resistance and virulence mechanisms that lead to treatment failure and increased mortality rates. Although for years the main driver of carbapenem resistance in Italy has been the Klebsiella pneumoniae KPC carbapenemase, recent years have seen an increase in VIM and NDM metallo-beta-lactamases (MBLs). We conducted a five-year survey of New Delhi Metallo-beta-Lactamase (NDM)-producing Klebsiella pneumoniae (NDM-Kpn) clinical isolates from the Lazio region, Italy; the study aimed to elucidate the molecular mechanisms underpinning their resistant and virulent phenotype. Methods: Antimicrobial susceptibility was evaluated by automated systems and broth microdilution. In silico analysis of acquired resistance and virulence genes was performed using whole-genome sequencing (WGS), molecular typing through MLST, and core genome multi-locus sequence typing (cgMLST). Conclusions: A total of 126 clinical NDM-Kpn isolates were collected from 19 distinct hospitals in the Lazio region. Molecular analysis highlighted the existence of NDM-1 (108/126) and NDM-5 (18/126) variants, 18 Sequence Types (STs), and 15 Cluster Types (CTs). Notably, 31/126 isolates displayed a virulence score of 4, carrying ybt , ICEKp , iuc , and rmp genes. This study identified a variety of NDM-Kpn STs, mainly carrying the bla |
| Databáze: | MEDLINE |
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