Impact of Exogenous Lactiplantibacillus plantarum on the Gut Microbiome of Hematopoietic Stem Cell Transplantation Patients Colonized by Multidrug-Resistant Bacteria: An Observational Study.

Autor: Moraes BDGC; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil.; Department of Hematology, Hemotherapy and Cell Therapy Service, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-000, SP, Brazil., Martins RCR; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil., Fonseca JVDS; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil., Franco LAM; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil., Pereira GCO; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil., Bartelli TF; Centro Internacional de Pesquisa, CIPE, A.C.Camargo Cancer Center, Rua Taguá 440, São Paulo 01508-010, SP, Brazil., Cortes MF; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil., Scaccia N; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil., Santos CF; Department of Hematology, Hemotherapy and Cell Therapy Service, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-000, SP, Brazil., Musqueira PT; Department of Hematology, Hemotherapy and Cell Therapy Service, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-000, SP, Brazil., Otuyama LJ; Department of Hematology, Hemotherapy and Cell Therapy Service, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-000, SP, Brazil., Pylro VS; Department of Biology, Federal University of Lavras, Lavras 37200-000, MG, Brazil.; Microbial Ecology and Bioinformatics, Biology Department, Federal University of Lavras-UFLA, Lavras 37200-900, MG, Brazil., Mariano L; Department of Hematology, Hemotherapy and Cell Therapy Service, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-000, SP, Brazil., Rocha V; Department of Hematology, Hemotherapy and Cell Therapy Service, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-000, SP, Brazil., Witkin SS; Division of Immunology and Infectious Diseases, Weill Cornell Medicine of Cornell University, New York, NY 10065, USA., Sabino E; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil., Guimaraes T; Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-000, SP, Brazil., Costa SF; LIM-46 and LIM49, Department of Infectious Diseases, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470 Jardim America, São Paulo 05403-000, SP, Brazil.
Jazyk: angličtina
Zdroj: Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2024 Oct 28; Vol. 13 (11). Date of Electronic Publication: 2024 Oct 28.
DOI: 10.3390/antibiotics13111010
Abstrakt: Background: Lactiplantibacillus plantarum can inhibit the growth of multidrug-resistant organisms (MDROs) and modulate the gut microbiome. However, data on hematopoietic stem cell transplantation (HSCT) are scarce. Aim: In an observational study, we assessed the impact of L. plantarum on the modulation of the gut microbiome in HSCT patients colonized by MDROs. Methods: Participants were allocated to an intervention group (IG = 22) who received capsules of L. plantarum (5 × 10 9 CFU) twice per day until the onset of neutropenia or a control group (CG = 20). The V4 region of the 16S bacterial rRNA gene was sequenced in 87 stool samples from a subset of 33 patients (IG = 20 and CG = 13). The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) program was used to predict metagenome functions. Results: L. plantarum demonstrated an average 86% (±11%) drug-target engagement at 43 (±29) days of consumption and was deemed safe, well-tolerated, and associated with an increase in the abundance of the Lactobacillales ( p < 0.05). A significant increase in Lactococcus and a reduction in Turicibacter ( p < 0.05) were identified on the second week of L. plantarum use. Although Enterococcus abundance had a greater rise in the CG ( p = 0.07), there were no significant differences concerning the Gram-negative MDROs. No serious adverse effects were reported in the IG. We observed a greater, non-significant pyruvate fermentation to propanoate I ( p = 0.193) relative abundance in the IG compared with the CG. L. plantarum use was safe and tolerable by HSCT patients. Conclusions: While L. plantarum is safe and may impact Enterococcus and Turicibacter abundance, it showed no impact on Gram-negative MDRO abundance in HSCT patients.
Databáze: MEDLINE
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