Immunomodulatory role of Trichinella spiralis-derived antigen on imiquimod-induced psoriasis in mice model.

Autor: El Skhawy N; Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt. nahla.elskhawy@alexmed.edu.eg., Eissa MM; Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt., Allam M; Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt., Eleryan EM; Department of Dermatology and Venereology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2024 Nov 27; Vol. 123 (11), pp. 397. Date of Electronic Publication: 2024 Nov 27.
DOI: 10.1007/s00436-024-08415-7
Abstrakt: The immunomodulatory activity of parasites has been extensively investigated in multiple immune-related diseases. However, dermatological diseases have been off the list for a long time despite their vast incidence and the deleterious consequences of some of them. This study explored the immunomodulatory role of autoclaved Trichinella spiralis (T. spiralis) larvae antigen (ATSLA) as a psoriasis immunotherapeutic candidate in a mice model. Psoriasis was induced in Swiss albino mice using commercial imiquimod cream (IMQ). Mice were randomly divided into the IMQ untreated control group and the IMQ treated group that was treated with ATSLA twice, on day 0 and day 3. Additional mice served as normal controls. Assessment of skin thickness, erythema, and scales was recorded. Total skin scores were calculated. Skin MDA levels, splenic indices, serum and skin IL-23, and tumor necrosis factor alpha (TNF-α) were measured. Skin sections were stained with H&E and immune stained for CD68-positive cells using immunohistochemistry. Treatment with ATSLA significantly reduced skin thickness, erythema, scales, and total skin scores in the IMQ-treated group compared to the untreated control. This was accompanied by a reduction in the splenic index, skin MDA levels, IL-23, and TNF-α in both the skin and serum of the treated group. Pathologically, skin sections of the treated group showed less epidermal thickness, acanthosis, hyperkeratosis, and CD68 cell count. The study concluded the immunotherapeutic activity of ATSLA in experimental psoriatic skin lesions. This will enrich the psoriasis immunotherapeutic list with novel candidates of parasitic origin.
Competing Interests: Declarations. Ethics approval: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures and animal treatments complied with the ARRIVE guidelines for the care and use of laboratory animals and were approved by the Institutional Animal Care and Use Committee in the Faculty of Medicine, Alexandria University (IACUC, 0306233). Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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