Fungus-derived opine enhances plant photosynthesis.
| Autor: | Kong D; College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China., Cui L; State Key Laboratory of Microbial Metabolism, Joint International Laboratory of Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China., Wang X; State Key Laboratory of Microbial Metabolism, Joint International Laboratory of Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China., Wo J; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China. Electronic address: wojing0330@zjut.edu.cn., Xiong F; College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China. Electronic address: xiongfj2021@swu.edu.cn. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of advanced research [J Adv Res] 2024 Nov 25. Date of Electronic Publication: 2024 Nov 25. |
| DOI: | 10.1016/j.jare.2024.11.029 |
| Abstrakt: | Introduction: Plant-fungal interactions stimulate endophytic fungi to produce a plethora of metabolites that enhance plant growth and improve stress resistance. Opines, naturally occurring compounds formed through the condensation of amino acids with α-keto acids or sugars, have diverse biological functions and are mainly present in bacteria. Interestingly, investigations have revealed the presence of opine synthases (OSases) in fungal species as well, and their functions are yet to be studied. Objectives: The objective of this study is to investigate the occurrence of OSases in fungal species, identify their products, and characterize the potential biological activity of the metabolites. Methods: We identified a putative class of OSases in fungi through sequence similarity network (SSN) analysis. The function of these enzymes was elucidated using methods including protein heterologous expression, in vitro biochemical characterization, in vivo gene knock-out, as well as product isolation and identification. Additionally, we conducted plant activity testing on the secondary metabolites through foliar spraying and performed transcriptomic analysis to uncover their functions. Results: A quarter of the PF18631 family members, which contain the C-terminal helical bundle domain of cucumopine synthase, are derived from endophytic fungi. Some of these enzymes catalyze the synthesis of tryptopine A (1-acetyl-3-carboxy-β-carboline) by condensing L-tryptophan and methylglyoxal. The tryptopine A can act as a growth regulator, promoting plant growth and transcriptionally reprogramming photosynthesis-related pathways, while enhancing the rate of plant photosynthesis by 25%. Conclusion: The findings of this study suggest that tryptopine A plays a crucial role as a signaling molecule in the establishment and maintenance of mutualistic associations between endophytic fungi and host plants, thereby broaden our comprehension of fungal-plant symbiosis. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier B.V.) |
| Databáze: | MEDLINE |
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