Biochemical mechanism underlying the synthesis of PbS nanoparticle and its in-situ photo effect on Shinella zoogloeoides PQ7.

Autor: Xin Y; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China; Department of Resources Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China., Huang C; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China., Zeng J; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China., Zhang W; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China., Zhou Y; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China., Xu Y; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China., Huang Y; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China. Electronic address: yilihuang@zju.edu.cn.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Nov 24; Vol. 482, pp. 136652. Date of Electronic Publication: 2024 Nov 24.
DOI: 10.1016/j.jhazmat.2024.136652
Abstrakt: Metal sulfide nanoparticles (NPs) with semiconductor potentials are valuable bioremediation end-products that attract great research interests. However, biochemical mechanisms underlying their biosynthesis and photo-effects remain elusive. In this study, we found that biofilm lifestyle remarkably improved lead resistance and PbS-NP biosynthesis in Shinella zoogloeoides PQ7. Surprisingly, biosynthesis of PbS-NP required more than cysteine and H 2 S production. Transcriptomic and metabolomic analysis indicated that PQ7 responded to lead stress by changing metabolic activities in ABC transporters, oxidative phosphorylation, EPS production, quorum sensing, protein de novo synthesis, flagella assembly and antioxidative reactions, etc. The elevated EPS production and quorum sensing gene expression echoed the favorable roles of biofilm formation in lead resistance. Biosynthesis of PbS-NP required proper oxygen supply, and was impeded by adding kanamycin or using yeast extract as the sole nutrient supply. Investigations on NAD/NADH, ATP, ROS and GSH productions indicated that biosynthesis of PbS-NP was corelated with cellular respiration, energy metabolism, and redox status. Finally, we proved that PbS-NP had the dose-dependent in-situ photo effect on PQ7's growth and ROS production. This is the first report that pinpoints the role of cellular respiration in PbS-NP biosynthesis, which is essential for further mechanism study and the development of bioremediation techniques.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Yili Huang reports financial support was provided by Zhejiang Provincial Natural Science Foundation of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Databáze: MEDLINE
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