| Autor: |
Luca A; Department of Biomedical Sciences, Faculty of Medical Bioengineering, 'Grigore T. Popa' University of Medicine and Pharmacy, 700115 Iasi, Romania., Cojocaru FD; Department of Biomedical Sciences, Faculty of Medical Bioengineering, 'Grigore T. Popa' University of Medicine and Pharmacy, 700115 Iasi, Romania., Pascal MS; Department of Biomedical Sciences, Faculty of Medical Bioengineering, 'Grigore T. Popa' University of Medicine and Pharmacy, 700115 Iasi, Romania., Vlad T; Department of Biomedical Sciences, Faculty of Medical Bioengineering, 'Grigore T. Popa' University of Medicine and Pharmacy, 700115 Iasi, Romania., Nacu I; Department of Biomedical Sciences, Faculty of Medical Bioengineering, 'Grigore T. Popa' University of Medicine and Pharmacy, 700115 Iasi, Romania.; 'Petru Poni' Institute of Macromolecular Chemistry, 41 A Grigore Ghica Voda Alley, 700487 Iasi, Romania., Peptu CA; Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iaşi, 700050 Iasi, Romania., Butnaru M; Department of Biomedical Sciences, Faculty of Medical Bioengineering, 'Grigore T. Popa' University of Medicine and Pharmacy, 700115 Iasi, Romania., Verestiuc L; Department of Biomedical Sciences, Faculty of Medical Bioengineering, 'Grigore T. Popa' University of Medicine and Pharmacy, 700115 Iasi, Romania. |
| Abstrakt: |
Due to their indisputable biocompatibility and abundant source, biopolymers are widely used to prepare hydrogels for skin tissue engineering. Among them, cellulose is a great option for this challenging application due to its increased water retention capacity, mechanical strength, versatility and unlimited availability. Since algae are an unexploited source of cellulose, the novelty of this study is the decellularization of two different species, freshly collected from the Black Sea coast, using two different chemical surfactants (sodium dodecyl sulphate and Triton X-100), and characterisation of the resulted complex biopolymeric 3D matrices. The algae nature and decellularization agent significantly influenced the matrices porosity, while the values obtained for the hydration degree included them in hydrogel class. Moreover, their capacity to retain and then controllably release an anti-inflammatory drug, ibuprofen, led us to recommend the obtained structures as drug delivery systems. The decellularized macroalgae hydrogels are bioadhesive and cytocompatible in direct contact with human keratinocytes and represent a great support for cells. Finally, it was noticed that human keratinocytes (HaCaT cell line) adhered and populated the structures during a monitoring period of 14 days. |
