Maize unstable factor for orange1 encodes a nuclear protein that affects redox accumulation during kernel development.

Autor:	Chatterjee D; Department of Plant Science, The Pennsylvania State University, University Park, PA 16802, USA., Zhang Z; National Center for Maize Improvement, China Agricultural University, Beijing 100083, China., Lin PY; Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan., Wang PH; Department of Plant Science, The Pennsylvania State University, University Park, PA 16802, USA., Sidhu GK; Department of Plant Science, The Pennsylvania State University, University Park, PA 16802, USA., Yennawar NH; X-Ray Crystallography Facility, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Hsieh JA; Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.; Genome and Systems Biology Degree Program, Academia Sinica and National Taiwan University, Taipei 10617, Taiwan., Chen PY; Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.; Genome and Systems Biology Degree Program, Academia Sinica and National Taiwan University, Taipei 10617, Taiwan., Song R; National Center for Maize Improvement, China Agricultural University, Beijing 100083, China., Meyers BC; The Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.; Division of Plant Science and Technology, University of Missouri, Columbia, MO 65201, USA., Chopra S; Department of Plant Science, The Pennsylvania State University, University Park, PA 16802, USA.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Nov 26. Date of Electronic Publication: 2024 Nov 26.
DOI:	10.1093/plcell/koae301
Abstrakt:	The basal endosperm transfer layer (BETL) of the maize (Zea mays L.) kernel is composed of transfer cells for nutrient transport to nourish the developing kernel. To understand the spatiotemporal processes required for BETL development, we characterized 2 unstable factor for orange1 (Zmufo1) mutant alleles. The BETL defects in these mutants were associated with high levels of reactive oxygen species, oxidative DNA damage, and cell death. Interestingly, antioxidant supplementation in in vitro cultured kernels alleviated the cellular defects in mutants. Transcriptome analysis of the loss-of-function Zmufo1 allele showed differential expression of tricarboxylic acid cycle, redox homeostasis, and BETL-related genes. The basal endosperms of the mutant alleles had high levels of acetyl-CoA and elevated histone acetyltransferase activity. The BETL cell nuclei showed reduced electron-dense regions, indicating sparse heterochromatin distribution in the mutants compared with wild-type. Zmufo1 overexpression further reduced histone methylation marks in the enhancer and gene body regions of the pericarp color1 (Zmp1) reporter gene. Zmufo1 encodes an intrinsically disordered nuclear protein with very low sequence similarity to known proteins. Yeast two-hybrid and luciferase complementation assays established that ZmUFO1 interacts with proteins that play a role in chromatin remodeling, nuclear transport, and transcriptional regulation. This study establishes the critical function of Zmufo1 during basal endosperm development in maize kernels. Competing Interests: Conflict of interest statement: The authors declare no competing financial interests. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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