Exploring the molecular interactions between nephrolithiasis and carotid atherosclerosis: asporin as a potential biomarker.

Autor: Hua Y; Department of Urology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu, 210029, P.R. China., Zhou Z; Department of Urology, Huashan Hospital, Fudan University, Shanghai, 200040, PR China., Miao S; Department of Vascular Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu, 210029, P.R. China., Wang Z; Department of Urology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu, 210029, P.R. China., Song R; Department of Urology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu, 210029, P.R. China. songrijin@163.com., Meng X; Department of Urology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu, 210029, P.R. China. xhmeng@njmu.edu.cn.
Jazyk: angličtina
Zdroj: Urolithiasis [Urolithiasis] 2024 Nov 26; Vol. 52 (1), pp. 169. Date of Electronic Publication: 2024 Nov 26.
DOI: 10.1007/s00240-024-01665-1
Abstrakt: Increasing evidence suggested nephrolithiasis has a close linkage with carotid atherosclerosis (CAS), with Randall's plaque (RP) being a precursor to kidney stones. Our study aimed to examine the crosstalk genes and potential molecular mechanisms between RP and CAS. We obtained microarray data for RP and CAS from the Gene Expression Omnibus (GEO) and used weighted gene co-expression network analysis (WGCNA) and differential gene expression (DEG) analysis to identify shared genes. By integrating WGCNA and DEG analysis, Asporin (ASPN) was identified as the key gene connecting RP and CAS, with its diagnostic potential assessed via a receiver operating characteristic (ROC) curve. Immune infiltration studies showed a significant correlation between ASPN and various immune cells in RP and CAS. ASPN was found to be less expressed in RP and CAS tissues compared to normal tissues, as confirmed by immunohistochemistry (IHC) and quantitative reverse-transcription PCR (qRT-PCR). The rat model confirmed the human tissue findings. ASPN can elucidate the shared pathogenic mechanisms underlying the two conditions, including immune response and osteoblast differentiation.
Competing Interests: Declarations. Ethical approval: Approval of ethics and agreement to join The Ethics Committee of the First Affiliated Hospital of Nanjing Medical University granted ethical approval for this research, under reference number 2022-SRFA-429. This study’s employment of animal subjects was evaluated and sanctioned by the Institutional Animal Care and Use Committee at Nanjing Medical University. Consent for publication: All participants provided their written consent after being informed. Conflict of interests: The writers state that they possess no conflicting interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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