| Autor: |
Yu H; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. weibingzhang@ecust.edu.cn., Qiang Z; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. weibingzhang@ecust.edu.cn., Sun Y; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. weibingzhang@ecust.edu.cn., Sun M; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. weibingzhang@ecust.edu.cn., Zhang L; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. weibingzhang@ecust.edu.cn., Yu B; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. weibingzhang@ecust.edu.cn., Lei W; Shanghai Research Institute of Chemical Industry Co., Ltd., 345 East Yunling Road, Shanghai, 200062, China. leiwensrici@163.com., Zhang W; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. weibingzhang@ecust.edu.cn. |
| Abstrakt: |
Based on the specific hydrolytic ability of alkaline phosphatase (ALP), a novel biocompatible ratiometric lanthanide fluorescent probe based on an internal reference (CIP@SiO 2 -Ce/ATP-Tris) was constructed with Ce 3+ as the central ion, adenosine triphosphate (ATP) as the ligand, Tris as the auxiliary ligand and ciprofloxacin (CIP) encapsulated into SiO 2 nanoparticles as the reference signal. The fluorescent probe emits characteristic fluorescence at 363 nm belonging to Ce 3+ as the working signal and at 435 nm belonging to CIP as the reference signal. Dephosphorylation disrupted the coordination of Ce/ATP-Tris with the enzymatic reaction of ALP, which resulted in fluorescence quenching of Ce 3+ . The reference fluorescence was kept stable because of the protective effect of encapsulation by SiO 2 . The biosensor analysis method was achieved by comparing the relationship between I 435 / I 363 and ALP concentration. The detection limit is 0.0025 U L -1 , and the linear range of detection is 0.1-20 U L -1 . It was subsequently used to detect ALP in samples of fetal bovine serum and human serum, and promising results were obtained. |
