The lives of cells, recorded.

Autor: Askary A; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA., Chen W; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Choi J; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Du LY; Biozentrum, University of Basel, Basel, Switzerland.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA., Elowitz MB; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA. melowitz@caltech.edu.; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. melowitz@caltech.edu.; Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA, USA. melowitz@caltech.edu., Gagnon JA; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA. james.gagnon@utah.edu., Schier AF; Biozentrum, University of Basel, Basel, Switzerland. alex.schier@unibas.ch.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA. alex.schier@unibas.ch., Seidel S; Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.; Swiss Institute of Bioinformatics, Lausanne, Switzerland., Shendure J; Department of Genome Sciences, University of Washington, Seattle, WA, USA. shendure@uw.edu.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA. shendure@uw.edu.; Howard Hughes Medical Institute, Seattle, WA, USA. shendure@uw.edu.; Brotman Baty Institute for Precision Medicine, University of Washington, Seattle, WA, USA. shendure@uw.edu.; Seattle Hub for Synthetic Biology, Seattle, WA, USA. shendure@uw.edu., Stadler T; Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland. tanja.stadler@bsse.ethz.ch.; Swiss Institute of Bioinformatics, Lausanne, Switzerland. tanja.stadler@bsse.ethz.ch., Tran M; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Jazyk: angličtina
Zdroj: Nature reviews. Genetics [Nat Rev Genet] 2024 Nov 25. Date of Electronic Publication: 2024 Nov 25.
DOI: 10.1038/s41576-024-00788-w
Abstrakt: A paradigm for biology is emerging in which cells can be genetically programmed to write their histories into their own genomes. These records can subsequently be read, and the cellular histories reconstructed, which for each cell could include a record of its lineage relationships, extrinsic influences, internal states and physical locations, over time. DNA recording has the potential to transform the way that we study developmental and disease processes. Recent advances in genome engineering are driving the development of systems for DNA recording, and meanwhile single-cell and spatial omics technologies increasingly enable the recovery of the recorded information. Combined with advances in computational and phylogenetic inference algorithms, the DNA recording paradigm is beginning to bear fruit. In this Perspective, we explore the rationale and technical basis of DNA recording, what aspects of cellular biology might be recorded and how, and the types of discovery that we anticipate this paradigm will enable.
Competing Interests: Competing interests: A.A., W.C., J.C., M.B.E. and J.S. have patents related to DNA-based molecular recording. J.S. is a scientific advisory board member, consultant and/or co-founder of Cajal Neuroscience, Guardant Health, Maze Therapeutics, Camp4 Therapeutics, Phase Genomics, Adaptive Biotechnologies, Scale Biosciences, Sixth Street Capital, Prime Medicine, Somite Therapeutics and Pacific Biosciences. M.B.E. is a scientific advisory board member, consultant and/or co-founder of Primordium Labs, TeraCyte, Spatial Genomics, and Asymptote Genetic Medicines. The other authors declare no competing interests.
(© 2024. Springer Nature Limited.)
Databáze: MEDLINE
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