Sample loading in gel electrophoresis using adapted 3D printers.

Autor: Tao T; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia., Kopyl A; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia., Yew Y; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia., El-Dani F; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia., Abid HA; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia; Department of Aerospace Engineering, King Fahd University of Petroleum & Minerals (KFUPM), Dhahran, 31261, Saudi Arabia., Hiscox T; School of Biological Sciences, Monash University, VIC, 3800, Australia., Liew OW; Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Centre for Translational Medicine, 14 Medical Drive, 117599, Singapore., Ng TW; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, 3800, Australia. Electronic address: engngtw@gmail.com.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2025 Feb; Vol. 697, pp. 115721. Date of Electronic Publication: 2024 Nov 23.
DOI: 10.1016/j.ab.2024.115721
Abstrakt: In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.
Competing Interests: Declaration of competing interest The authors have no interests to declare.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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