Effects of environmentally relevant concentrations of glyphosate and aminomethylphosphonic acid on biotransformation and stress response proteins in the liver of zebrafish (Danio rerio).

Autor: Morozov AA; Papanin Institute for Biology of Inland Waters Russian Academy of Sciences, IBIW RAS, 109, Borok 152742, Russia. Electronic address: aleksey.a.morozov@gmail.com., Yurchenko VV; Papanin Institute for Biology of Inland Waters Russian Academy of Sciences, IBIW RAS, 109, Borok 152742, Russia.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part D, Genomics & proteomics [Comp Biochem Physiol Part D Genomics Proteomics] 2024 Nov 22; Vol. 53, pp. 101366. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1016/j.cbd.2024.101366
Abstrakt: Herbicides pose a threat to various non-target organisms, including fish. A widely used herbicide, glyphosate, and its main breakdown product, aminomethylphosphonic acid (AMPA), are quite ubiquitous in freshwater systems. The aim of this work was to analyze changes in the relative abundance of hepatic proteins participating in the biotransformation and response to chemical stress in adult zebrafish Danio rerio exposed to environmentally relevant concentrations of glyphosate (100 μg/L), AMPA (100 μg/L), and their mixture (50 μg/L + 50 μg/L) for two weeks. Proteomic analysis showed that the tested concentrations caused dysregulation of various biotransformation proteins, the most upregulated of which in all treatment groups was the Phase I enzyme cyp27a7. While glyphosate had a more pronounced impact on the biotransformation pathways, AMPA showed stronger interference with redox homeostasis. When acting together, the parent compound and its metabolite were more potent to disturb fish metabolic processes, including nucleotide metabolism and proteasome pathway, and to downregulate proteins known for their roles in protection from oxidative modifications of cellular constituents and disruption of redox signaling.
Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have influenced the research results reported in this study.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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