In Vivo Test to Evaluate the Cytotoxicity and Genotoxicity of Natural Zeolite (NZ) Functionalized with Silver Nanoparticles (NZ-AgNPs) on Erythrocytes of Yellowtail Tetra Fish Astyanax lacustris.

Autor: da Silva JRR; Graduate Program of Environmental Biotechnology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil. josianerrs@hotmail.com., de Andrade CO; Graduate Program of Environmental Biotechnology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil., Ribeiro AC; Graduate Program of Environmental Biotechnology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil.; Linking Landscape, Environment, Agriculture and Food, School of Agriculture - University of Lisbon, Lisbon, Portugal., Macruz PD; Department of Chemical, Biomolecules and Mass Spectrometry Laboratory, State University of Maringá, Maringá, Paraná, Brazil., da Costa Neves FernandesAlmeida Duarte E; Linking Landscape, Environment, Agriculture and Food, School of Agriculture - University of Lisbon, Lisbon, Portugal., Bergamasco R; Department of Chemical Engineering, State University of Maringá, Maringá, Paraná, Brazil., de Brito Portela-Castro AL; Graduate Program of Environmental Biotechnology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil.; Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maringá, Maringá, Paraná, Brazil., Fernandes CA; Graduate Program of Environmental Biotechnology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil.; Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maringá, Maringá, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2024 Nov 25. Date of Electronic Publication: 2024 Nov 25.
DOI: 10.1007/s12011-024-04451-7
Abstrakt: Silver nanoparticles (AgNPs) have unique properties such as chemical stability, malleability, flexibility, high electrical and thermal conductivity, and catalytic activity, making them suitable for various applications. However, they also pose potential toxicity and environmental contamination risks. Natural zeolites (NZs) are considered excellent inorganic support for reducing the toxicity of AgNPs. The scope of this study was to carry out a comparative assessment of the cytotoxic and genotoxic effects of NZ, NZ functionalized with silver nanoparticles (NZ-AgNPs), and commercial AgNPs on fish Astyanax lacustris. Micronucleus (MN) test, cellular morphological change (CMC) test, and the comet assay were used to assess the effects of the nanoparticles. A. lacustris specimens were exposed to a concentrations of 0.1 mg l -1 in the NZs, NZ-AgNPs, and commercial AgNPs for 96 h during the experiment. The results showed no significant difference between the negative control and NZ. Moreover, NZ-AgNPs exhibited reduced toxicities compared to commercial AgNPs, which caused higher levels of alterations and cellular damage. The study concluded that NZs effectively reduced cytotoxicity/genotoxicity in A. lacustris specimens when used to support AgNPs.
Competing Interests: Declarations. Ethical Approval: The ethics committee approved all procedures for using animals in research (CEUA-UEM), with license no. 3062160921. Consent for Publication: All authors have agreed for publication. Competing Interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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