Prevalence of culicine salivary antibodies in non-human primates living in national parks in Thailand.

Autor: Aguila AMA; Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Boonnak K; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand., Tongthainan D; Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok, Chonburi, Thailand., Reamtong O; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Suthisawat S; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand., Likhit O; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand., Fungfuang W; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand., Hii J; College of Public Health, Medical and Veterinary Sciences, James Cook University, North Queensland, Queensland, Australia., Sriwichai P; Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Jazyk: angličtina
Zdroj: Medical and veterinary entomology [Med Vet Entomol] 2024 Nov 25. Date of Electronic Publication: 2024 Nov 25.
DOI: 10.1111/mve.12779
Abstrakt: Macaques are widely distributed in Thailand with remarkable adaptation to living close to humans in residential, religious sites, markets and tourist areas. They play an essential role in the persistence of pathogens in the environment. As reservoir hosts, they are exposed to hematophagous vectors that secrete saliva, a cocktail of bioactive molecules including antigenic components stimulating host antibody production. Subsequent to the detection of mosquito-borne pathogens in macaques living in national parks, we aimed to determine the seroprevalence of antibodies to crude salivary gland extracts (SGEs) from culicine mosquitoes (Aedes aegypti [Linnaeus, 1762], Ae. albopictus [Skuse, 1895] and Culex quinquefasciatus [Say, 1823]) and compare individual titres between macaque species/national parks (33, Macaca arctoides [I. Geoffroy Saint-Hilaire, 1831] [Primates: Cercopithecidae] from Kaeng Krachan, 23 M. leonina leonina [Blyth, 1863] [Primates: Cercopithecidae] from Khao Yai and four M. fascicularis [Raffles, 1821] [Primates: Cercopithecidae] from Mu Ko Ranong). The anti-mosquito SGE antibodies found in 60 macaques from three national parks indicate varying levels of host-vector exposure. Macaque antibody titres were high against culicine mosquitoes. However, the significant difference among national parks (or macaque species) was only observed against Cx. quinquefasciatus. Correlation analysis of titres between Aedes SGE and arboviruses revealed a significantly more intense immune response against Ae. albopictus in DENV3-positive M. arctoides. Current findings support the concept of salivary biomarkers using accessible SGE, available from mosquito colonies of interest. However, we observed cross-reactivity between Aedes species because of crude SGE containing species-shared proteins. Nevertheless, a potential risk of pathogen transmission is emphasised between national park visitors and macaques via mosquitoes as bridge vectors. This information contributes to preventive measures against mosquito bites, including those implemented in tourist areas.
(© 2024 The Author(s). Medical and Veterinary Entomology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.)
Databáze: MEDLINE
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