Rapid curing dynamics of PEG-thiol-ene resins allow facile 3D bioprinting and in-air cell-laden microgel fabrication.

Autor:	K Jang L; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America., T Ahlquist J; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America., Ye C; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America., Trujillo J; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, United States of America., Triplett M; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America., L Moya M; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America., Robertson C; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America.; UC Davis Comprehensive Cancer Center, Davis, CA, United States of America., Hynes W; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America., M Wasson E; Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States of America.; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States of America.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Nov 25; Vol. 20 (1). Date of Electronic Publication: 2024 Nov 25.
DOI:	10.1088/1748-605X/ad8540
Abstrakt:	Thiol-norbornene photoclick hydrogels are highly efficient in tissue engineering applications due to their fast gelation, cytocompatibility, and tunability. In this work, we utilized the advantageous features of polyethylene glycol (PEG)-thiol-ene resins to enable fabrication of complex and heterogeneous tissue scaffolds using 3D bioprinting and in-air drop encapsulation techniques. We demonstrated that photoclickable PEG-thiol-ene resins could be tuned by varying the ratio of PEG-dithiol to PEG norbornene to generate a wide range of mechanical stiffness (0.5-12 kPa) and swelling ratios. Importantly, all formulations maintained a constant, rapid gelation time (<0.5 s). We used this resin in biological projection microstereolithography (BioP µ SL) to print complex structures with geometric fidelity and demonstrated biocompatibility by printing cell-laden microgrids. Moreover, the rapid gelling kinetics of this resin permitted high-throughput fabrication of tunable, cell-laden microgels in air using a biological in-air drop encapsulation apparatus (BioIDEA). We demonstrated that these microgels could support cell viability and be assembled into a gradient structure. This PEG-thiol-ene resin, along with BioP µ SL and BioIDEA technology, will allow rapid fabrication of complex and heterogeneous tissues that mimic native tissues with cellular and mechanical gradients. The engineered tissue scaffolds with a controlled microscale porosity could be utilized in applications including gradient tissue engineering, biosensing, and in vitro tissue models. (Creative Commons Attribution license.)
Databáze:	MEDLINE
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