Molecular identification of methane-consuming bacteria in the Persian Gulf: a study for microbial gas exploration.
| Autor: | Harirforoush M; Department of Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Shavandi M; Microbiology and Biotechnology Group, Environment and Biotechnology Research Division, Research Institute of Petroleum Industry, Tehran, Iran., Amoozegar MA; Extremophiles Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran., Saffarian P; Department of Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Hasrak S; Genome Center, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2024 Nov 08; Vol. 15, pp. 1435037. Date of Electronic Publication: 2024 Nov 08 (Print Publication: 2024). |
| DOI: | 10.3389/fmicb.2024.1435037 |
| Abstrakt: | The seepage of gaseous compounds from underground reservoirs towards the surface causes abnormalities in the population of microbial communities that consume light hydrocarbons on the surface of the reservoir. This microbial population can serve as indicators for determining the location of gas reservoirs prior to drilling operations. In this study, the simulation of methane gas leakage in the sediments of the Persian Gulf was conducted using a laboratory model. The objective of this simulation was to identify the microbial population consuming methane within the sediments of the Persian Gulf, aiding in the exploration of gas reserves. Continuous injection of methane gas into the system was performed for a period of 3 months to enrich the microbial consortia consuming methane. Subsequently, the microbial population was identified using next-generation sequencing (NGS) analysis. The results indicated that, based on the 16S rRNA sequencing dataset, aerobic methanotrophs, including genera Methylobacter , Methylomarinum , Methylomicrobium, Methylomonas , and Methylophage , were the dominant microbial group on the surface of the sediments. Additionally, anaerobic methane oxidation archaea in sediments were performed by ANME-2 and ANME-3 clades. The findings demonstrate that these microbial communities are capable of coexistence and thrive in long-term exposure to methane in the sediments of the Persian Gulf. Identifying this microbial pattern, alongside other geophysical and geological data, can increase the success rate of gas reservoir exploration. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Harirforoush, Shavandi, Amoozegar, Saffarian and Hasrak.) |
| Databáze: | MEDLINE |
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