Increasing pollination possibilities in Paspalum species: in vitro and in vivo viability of cryopreserved pollen to address flowering asynchrony.

Autor: Dinato NB; Center for Biological and Health Sciences, Federal University of São Carlos, Washington Luís, Km 235, São Carlos, SP 13565-905 Brazil., Vigna BBZ; Embrapa Southeastern Livestock, Brazilian Agricultural Research Corporation, Rodovia Washington Luís, Km 234, CP 339, São Carlos, SP 13560-970 Brazil., de Matta F; Embrapa Southeastern Livestock, Brazilian Agricultural Research Corporation, Rodovia Washington Luís, Km 234, CP 339, São Carlos, SP 13560-970 Brazil., Fávero AP; Embrapa Southeastern Livestock, Brazilian Agricultural Research Corporation, Rodovia Washington Luís, Km 234, CP 339, São Carlos, SP 13560-970 Brazil.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2024 Dec; Vol. 14 (12), pp. 308. Date of Electronic Publication: 2024 Nov 21.
DOI: 10.1007/s13205-024-04138-9
Abstrakt: Paspalum is a vital forage and turf grass in tropical and subtropical regions, yet its breeding programs face challenges due to the lack of natural flowering synchronization between some parent species. Pollen cryopreservation offers a potential solution to this issue. This study aimed to adapt a cryopreservation protocol for the pollen of P. atratum , P. malacophyllum , and P. regnellii , and to evaluate the viability of cryopreserved pollen grains (CPG) for hybridization purposes. Two dehydrating agents (LiCl and silica gel) were tested for different durations (30, 60, and 120 min) alongside a non-dehydration treatment. The effectiveness of cryopreservation was assessed over multiple time points (1, 10, 30, 90, 180, 270, and 365 days) with freshly harvested grains as controls. Pollen viability was determined using 0.25% 2,3,5-triphenyltetrazolium chloride staining. Viability of CPG ranged from 40.67 to 80.67% across treatments. Optimal dehydration involved LiCl for 30 min and silica gel for 120 min, achieving an average viability of 66% after 12 months, comparable to fresh pollen. In vivo germination tests confirmed successful pollen tube germination with the combinations P. urvillei  ×  P. malacophyllum ; P. urvillei  ×  P. regnellii and hybrid ( P. plicatulum 4PT ×  P. guenoarum cv. Azulão) ×  P. atratum , although pollen tubes did not reach the micropyle in some crosses. This study established effective pollen cryopreservation protocols for P. atratum and P. malacophyllum , facilitating in vivo germination and enhancing the potential for hybridization in Paspalum breeding programs, thereby addressing flowering asynchrony and broadening crossing opportunities within the genus.
Competing Interests: Conflict of interestOn behalf of all authors, the corresponding author declares that there is no conflict of interest.
(© King Abdulaziz City for Science and Technology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
Externí odkaz: