Association between genetic clades and cancer prevalence suggested by French-wide study of oncogenic small ruminant β-retrovirus diversity.

Autor:	Riocreux-Verney B; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France., Verneret M; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France.; Universite Claude Bernard Lyon 1, Laboratory of Biometry and Evolutionary Biology (LBBE), UMR 5558, Centre national de la recherche scientifique (CNRS), VetAgro Sup, Villeurbanne, France., Diesler R; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France., Dolmazon C; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France., Gineys B; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France., Cadoré JL; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France.; VetAgro Sup, Veterinary Campus of Lyon, Marcy L'Etoile, France., Turpin J; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France., Leroux C; Viral Infections and Comparative Pathology (IVPC) UMR754, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Universite Claude Bernard Lyon 1, École Pratique des Hautes Études (EPHE), Université Paris Sciences & Lettres (PSL), Lyon, France.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Nov 08; Vol. 14, pp. 1466333. Date of Electronic Publication: 2024 Nov 08 (Print Publication: 2024).
DOI:	10.3389/fcimb.2024.1466333
Abstrakt:	Introduction: ENTV (Enzootic Nasal Tumor Virus) and JSRV (Jaagsiekte Sheep Retrovirus) are β-retroviruses responsible for respiratory cancers in sheep and goats. In this study, we analyzed the genetic features of the sheep and goat β-Retroviruses (29 JSRV and 24 ENTV strains) circulating in France to identify molecular signatures associated with disease severity in flocks. Methods: We developed a highly specific PCR to amplify and sequence exogenous targeted regions or near full length proviruses based on limited discriminating motifs along their genomes. Results: The phylogenetic reconstructions based on the Long Terminal Repeat (LTR) and env regions suggest that one major strain is circulating on the French territory for ENTV-1 and ENTV-2 while not clustering with already published Spanish, Canadian or Chinese strains. JSRV strains circulating in French sheep flocks were distributed in 2 distinct genetic clades clustering with sequences originating from North America, Africa and United-Kingdom. JSRV clade I was found to be associated with a higher incidence of cancer in French flocks. Specific motifs spanning the entire JSRV genome particularly in the LTRs and in the intracytoplasmic domain of the envelope were detected between the two genetic subtypes. Discussion: This work represents the first nationwide study describing the circulation of the three closely related β-oncogenic retroviruses JSRV, ENTV-1 and ENTV-2 in French sheep and goat flocks. Better characterization of strain genetics is a critical step in monitoring circulating - retroviruses, especially those associated with higher cancer incidence in small ruminants. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Riocreux-Verney, Verneret, Diesler, Dolmazon, Gineys, Cadoré, Turpin and Leroux.)
Databáze:	MEDLINE
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