Catechin Hydrate Improves Hypertrophic Scar in Rabbit Ear Model via Reduction of Collagen Synthesis.
| Autor: | Goudarzi Afshar S; Department of Pharmacology & Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran., Tamri P; Department of Pharmacology & Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran., Nourian A; Department of Laboratory Sciences, Faculty of Para-Veterinary Sciences, Bu-Ali Sina University, Hamadan, Iran., Moahmoudi A; Department of Pharmacology & Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran. |
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| Jazyk: | angličtina |
| Zdroj: | Reports of biochemistry & molecular biology [Rep Biochem Mol Biol] 2024 Apr; Vol. 13 (1), pp. 13-22. |
| DOI: | 10.61186/rbmb.13.1.13 |
| Abstrakt: | Background: Hypertrophic scar (HS) is a cutaneous condition results from abnormal wound healing following deep tissue injury. To date, there is no optimal treatment for this skin disorder. Catechins possess anti-inflammatory, antioxidant and anti-fibrotic properties. In this study we investigated the effects of catechin hydrate (CH) in rabbit ear model of HS. Methods: A rabbit ear model of hypertrophic scar was set up. Ten New Zealand white rabbit were divided into 5 equal groups: non-treatment group, vehicle control, treated with intralesional injection of dimethyl sulfoxide (DMSO), and test groups, received intralesional injection of CH/DMSO solution at concentration of 0.25, 1.25 and, 2.5 mg/ml, respectively. The treatments were initiated 35 days after wounding once a week for 4 weeks. The scar elevation index (SEI) and the epidermal thickness index (ETI) were measured using Hematoxylin and Eosin (H & E) staining and the amount of collagen deposition were determined after Masson' trichrome staining. In addition, the enzyme-linked immunosorbent assay (ELISA) method was used to determine the levels of type І and ІІІ collagen and matrix metalloproteinase 1 (MMP1) in scar tissues. Results: CH improved abnormal scarring at concentrations of 1.25 and 2.5 mg/ml and significantly (P<0.001) reduced the SEI and ETI. The levels of collagen type І and type ІІІ, and total collagen deposition were significantly (P<0.05) decreased in scar tissues of CH treated groups and no significant effect on MMP1 levels. Conclusions: Our findings demonstrated that CH has the potential for the treatment of HSs. Competing Interests: The authors declare there is no conflict of interest. |
| Databáze: | MEDLINE |
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