Sulfated Cellulose Nanofiber Hydrogel with Mucus-Like Activities for Virus Inhibition.

Autor: Long Y; Institute for Chemistry und Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany., Dimde M; Institute for Chemistry und Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany.; Forschungszentrum für Elektronenmikroskopie und Gerätezentrum BioSupraMol, Freie Universität Berlin, Fabeckstraße 36A, 14195 Berlin, Germany., Adler JM; Institut für Virologie, Freie Universität Berlin, Robert von Ostertag-Str. 7-13, 14163, Berlin, Germany., Vidal RM; Institut für Virologie, Freie Universität Berlin, Robert von Ostertag-Str. 7-13, 14163, Berlin, Germany., Povolotsky TL; Institute for Chemistry und Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany., Nickl P; Institute for Chemistry und Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany., Achazi K; Institute for Chemistry und Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany., Trimpert J; Institut für Virologie, Freie Universität Berlin, Robert von Ostertag-Str. 7-13, 14163, Berlin, Germany., Kaufer BB; Institut für Virologie, Freie Universität Berlin, Robert von Ostertag-Str. 7-13, 14163, Berlin, Germany., Haag R; Institute for Chemistry und Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany., Nie C; Institute for Chemistry und Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany.
Jazyk: angličtina
Zdroj: ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2024 Nov 24. Date of Electronic Publication: 2024 Nov 24.
DOI: 10.1021/acsami.4c17998
Abstrakt: Mucus is the first defense barrier against viruses in the human immune system. Inspired by the mucus structure, we designed a highly sulfated hydrogel to bind viruses and prevent infection of the underlying cells. The hydrogel was formed by gelation of sulfated cellulose nanofiber (SCNF) with Ca 2+ . SCNF exhibited a mucin-like nanofiber structure with high numbers of sulfated groups. Based on the electrostatic interactions with a virus, SCNF could efficiently inhibit herpes simplex virus-1 (HSV-1) infection with a half-maximal inhibitory concentration (IC 50 ) of 0.43 μg/mL, which is comparable to that of heparin (IC 50 = 0.30 μg/mL). Benefiting from the multiporous structure and sulfate groups, the Ca 2+ -SCNF hydrogel could efficiently trap HSV-1 and inhibit the virus from attacking the underlying cells in a transwell model. Furthermore, SCNF also inhibited SARS-CoV-2 infection in a similar experimental setting. By integrating the advantages of high and broad-spectrum virus inhibitory activity as well as low toxicity, it is believed that the Ca 2+ -SCNF hydrogel can promote the development of highly biocompatible and efficient antiviral material with "binding and inhibition" capability and other diverse strategies.
Databáze: MEDLINE
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