Elevated CO 2 alters antibiotic resistome in soil amended with sulfamethazine via chemical-organic fertilization.

Autor: Xu F; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China; Institute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Nutritional Quality and Safety of Agro-products, Wuhan 430064, China., Xiang Q; Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, China., Xu ML; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China., Carter LJ; School of Geography, Faculty of Environment, University of Leeds, Leeds LS2 9JT, UK., Du WC; School of Environment, Nanjing Normal University, Nanjing 210023, China., Zhu CW; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China., Ai FX; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China., Yin Y; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China., Ji R; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China., Guo HY; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: hyguo@nju.edu.cn.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2024 Nov 22, pp. 120416. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1016/j.envres.2024.120416
Abstrakt: Rising antimicrobial resistance (AMR) is an enormous challenge for global healthcare systems. The effects of elevated CO 2 (eCO 2 ) on AMR are poorly characterized. Using a free-air CO 2 enrichment system and high-throughput qPCR arrays, we investigated the response of soil antibiotic resistome and bacterial communities to eCO 2 (ambient + 200 ppm) in soils amended with sulfamethazine (SMZ) at 0.1 and 1 mg kg -1 via chemical-organic fertilizer (COL, COH). Results showed that under ambient condition, COH significantly enhanced the diversity of high-risk antibiotic resistance genes (ARGs), relative abundance of low risk ARGs, unassessed ARGs and total ARGs compared to COL. Nevertheless, eCO 2 mitigated the effects of COH, with no significant difference found between COL and COH on the above high risk, low risk, unassessed and total ARGs. Meanwhile, eCO 2 decreased the relative abundance of spcN, ermA, olec, oprD, sulA-olP, tetB, tetT and vanXD in COL, and alleviated the enrichment of pikR2, ampC, lunC, oprD and pncA caused by the application of SMZ at 1 mg kg -1 . Correlation and network analysis illustrated that changes of certain bacteria biomarkers and horizontal gene transfer of integrase gene were associated with the altered response of ARGs abundance to eCO 2 . This study adds knowledge of the potential risk of antibiotic resistance in agricultural exposure scenarios under increasing CO 2 concentration.
Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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