Exposure of lung fibroblasts to PM 2.5 and lead (Pb) induces fibrosis and apoptosis in alveolar epithelial cells via a paracrine effect.

Autor: Choi JY; Department of Internal Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea., Kang M; Department of Internal Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea., Jung JH; Department of Internal Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea., Kim WJ; Department of Internal Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea; Department of Integrated Particulate Matter Management, Kangwon National University, Chuncheon, Republic of Korea., Yang HS; Respiratory Safety Research Center, Korea Institute of Toxicology, Jeongeup, Republic of Korea., Lee K; Respiratory Safety Research Center, Korea Institute of Toxicology, Jeongeup, Republic of Korea; Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon, Republic of Korea., Lee J; Department of Thoracic and Cardiovascular Surgery, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea., Yang SR; Department of Thoracic and Cardiovascular Surgery, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea; Department of Integrated Particulate Matter Management, Kangwon National University, Chuncheon, Republic of Korea., Rhee CK; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Republic of Korea., Hong SH; Department of Internal Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea; Department of Integrated Particulate Matter Management, Kangwon National University, Chuncheon, Republic of Korea; KW-Bio Co. Ltd., Chuncheon, Republic of Korea. Electronic address: shhong@kangwon.ac.kr.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2024 Dec; Vol. 288, pp. 117401. Date of Electronic Publication: 2024 Nov 23.
DOI: 10.1016/j.ecoenv.2024.117401
Abstrakt: Exposure to fine particulate matter (PM 2.5 ) and heavy metals (HMs) in the air is closely associated with the incidence and exacerbation of pulmonary fibrosis. Although the specific responses of alveolar epithelial cells (AECs) and lung fibroblasts to PM 2.5 or HM exposure have been well defined, the cellular responses of lung fibroblasts to PM 2.5 or HM exposure and the subsequent interactions with AECs remain poorly investigated. In this study, we demonstrated that human lung fibroblasts exposed to PM 2.5 or lead (Pb) induced fibrotic changes and apoptosis in AECs. Lung fibroblasts exposed to PM 2.5 induced fibrotic changes in AECs via a paracrine action. We further evaluated the detrimental effects of four HMs (cadmium, lead, arsenic, and manganese) present at the highest levels in the ambient air of South Korea, and investigated their paracrine effects on AECs. We found that long-term (14 passages) exposure to these HMs negatively affected the growth, migration, and survival of lung fibroblasts. Notably, manganese (Mn) significantly upregulated the expression of fibrotic markers with the activation of extracellular signal-regulated kinase (ERK) signaling in lung fibroblasts. However, treatment with conditioned medium (CM) collected from Mn-treated lung fibroblasts did not induce fibrotic changes in AECs. Interestingly, CM from Pb-treated lung fibroblasts significantly upregulated markers for fibrosis and apoptosis in AECs via activation of the ERK signaling pathway. These results suggest that understanding interactions between fibroblasts and AECs may provide useful strategies against PM or HM-induced injuries in alveolar tissue.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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