Physico-chemical properties of Priestia endophytica UCM B-5715 fluorescent pigments.

Autor: Kharkhota М; D.K. Zabolotny Institute of Microbiology and Virology of the NASU, Kyiv, Ukraine., Kharchuk М; D.K. Zabolotny Institute of Microbiology and Virology of the NASU, Kyiv, Ukraine., Kharchuk А; D.K. Zabolotny Institute of Microbiology and Virology of the NASU, Kyiv, Ukraine. Electronic address: alinapoliakova212@gmail.com., Grabova G; D.K. Zabolotny Institute of Microbiology and Virology of the NASU, Kyiv, Ukraine., Noskov Y; V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry (IBOPC) of the NASU, Kyiv, Ukraine., Linnik R; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine., Makeiev А; Taras Shevchenko National University of Kyiv, Kyiv, Ukraine., Avdieieva L; D.K. Zabolotny Institute of Microbiology and Virology of the NASU, Kyiv, Ukraine.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Dec 31; Vol. 741, pp. 151040. Date of Electronic Publication: 2024 Nov 20.
DOI: 10.1016/j.bbrc.2024.151040
Abstrakt: The endophytic bacterium Priestia endophytica (Bacillus endophyticus) UCM B-5715 (= DSM 13796) has been found to produce a distinctive pink pigment exhibiting vibrant yellow fluorescence. Investigation of the pigment extract revealed the presence of 2 non-polar fluorescent-colored compounds, with molecular masses of 376 (14.12 %) and 410 (82.02 %) a.m.u. FTIR spectroscopy indicated the characteristic signatures of heliomycin and chlorxanthomycin IR spectra, respectively. The chlorxathomycin nature of the main compound was confirmed by H 1 NMR spectroscopy. Light, luminescence, transmission electron microscopy, and IR and H 1 NMR spectroscopy established a high probability of a close association between the colored fluorescent compounds and poly-β-hydroxybutyrate granules. Bioinformatics analysis utilizing the antiSMASH 6.0 tool unveiled key gene sequences encoding the type II polyketide synthase complex and halogenase, involved in the biosynthesis of heliomycin and chlorxanthomycin.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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