Dual-channel fluorescent probe for detecting SO 2 and Hg 2+ , and dynamic imaging SO 2 under Cd 2+ in alfalfa (Medicago sativa).

Autor: Wu YQ; Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, 230012, China., Wang XY; Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, 230012, China., Bai TW; Key Laboratory of Medical Electronics and Digital Health of Zhejiang Province in Jiaxing University, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China. Electronic address: baitw@zjxu.edu.cn., Fu YL; Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, 230012, China; Anhui Biochem Pharmaceutical Co., Ltd., Taihe, 236699, China. Electronic address: ylfu@ahtcm.edu.cn.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Dec 15; Vol. 1332, pp. 343353. Date of Electronic Publication: 2024 Oct 19.
DOI: 10.1016/j.aca.2024.343353
Abstrakt: Background: As an antioxidant, SO 2 plays a critical role in maintaining the plant's redox balance. At the same time, Hg 2+ as heavy metals, will accumulate in plants through soil, often disrupting physiological processes in plants. However, few studies have been reported to examine changes in Hg 2+ and SO 2 in plants. Here, we developed a dual-channel fluorescent probe NYD, which can differentiate between the detection of Hg 2+ and SO 2 .
Results: The probe exhibits high sensitivity (LOD, 44 nM for Hg 2+ and 26 nM for SO 2 ). When Hg 2+ and HSO 3 - deprotect and attack the double bond by nucleophilic addition, significant emission of fluorescence at 475 nm and 574 nm with blue and red fluorescence respectively. In addition, NYD has the capability to detect the levels of Hg 2+ and SO 2 in cells and alfalfa seedlings. Meanwhile, the findings indicated that alfalfa seedlings were exposed to Cd 2+ , which led to redox imbalance and increased endogenous SO 2 production.
Significance: We anticipate that this research will contribute to a better understanding of the functions of SO 2 under oxidative stress induced by Cd 2+ .
Competing Interests: Declaration of competing interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Dual-channel fluorescent probe for detecting SO(2)and Hg(2+), and dynamic imaging SO(2)under Cd(2+)in alfalfa (Medicago sativa)”.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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