Top-down proteomics of venoms from five Micrurus species from Costa Rica: Comparative composition of phospholipase A 2 -rich vs three-finger toxin-rich phenotypes.

Autor: Fernández J; Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, 11501, Costa Rica., Chaves W; Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, 11501, Costa Rica., Vargas-Diaz D; Facultad de Microbiología, Universidad de Costa Rica, San José, 11501, Costa Rica., Petras D; Department of Biochemistry, University of California Riverside, 169 Aberdeen 17 Dr, Riverside, CA, 92507, USA; Interfaculty Institute of Microbiology and Infection Medicine, University of Tuebingen, Auf der Morgenstelle 24, 72076, Tuebingen, Germany., Lomonte B; Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, 11501, Costa Rica. Electronic address: bruno.lomonte@ucr.ac.cr.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Dec; Vol. 252, pp. 108187. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1016/j.toxicon.2024.108187
Abstrakt: Coralsnakes of the genus Micrurus include more than 80 species distributed in the American continent. They produce potent neurotoxic venoms acting at the neuromuscular junction and potentially leading to respiratory paralysis and death. The vast majority of proteins in coralsnake venoms belong to the three-finger toxin (3FTx) and the group I phospholipase A 2 (PLA 2 ) families. Previous studies using 'bottom-up' proteomic strategies have revealed a compositional dichotomy of toxin expression by which different Micrurus species display a predominance of either 3FTx or PLA 2 proteins in their venoms, possibly linked to the phylogeographic structure of the genus radiation. 'Top-down' proteomics (TDP) allows the direct analysis of intact proteins in a high resolution mass spectrometer, circumventing the limitations of the 'peptide-to-protein inference problem' inherent to the bottom-up approach. Here, we analyzed the venoms of five out of the six Micrurus species that inhabit Costa Rica, by using a TDP approach. Results unveil venom proteoforms that are shared between these species, and provide additional insights into the variable compositional complexity of these venoms and relationships to their 3FTx/PLA 2 dichotomy.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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