Development of point-of-care immunodiagnostic test for Taenia solium cysticercosis in pigs.

Autor: Manjunathachar HV; Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India; ICMR-National Institute of Traditional Medicine, Belagavi 590010, Karnataka, India. Electronic address: manjunathachar632@gmail.com., Saravanan BC; Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India. Electronic address: drbcsaravanan@gmail.com., Joshi C; Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India., Mohmad A; Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India; Department of Higher Education, Jammu and Kashmir, India., Aravind M; Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India., Jacob SS; Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, Karnataka, India., Sankar M; Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2025 Jan; Vol. 182, pp. 105466. Date of Electronic Publication: 2024 Nov 20.
DOI: 10.1016/j.rvsc.2024.105466
Abstrakt: Cysticercosis, caused by the larval stage of Taenia solium, is a major zoonotic parasitic disease with a global impact, heightened by immigration and transboundary activities. Recognizing its importance, the World Health Organization has prioritized eliminating taeniasis/cysticercosis by 2030, designating it as a priority neglected tropical disease (NTD). Accurate diagnosis is essential for effective disease control, yet traditional methods often fall short due to low sensitivity of the test and the need for specialized laboratory setups. This study focuses on developing a recombinant Ag2 (rAg2)-based dot blot assay to detect T. solium cysticercosis in pigs. The rAg2 antigen was successfully expressed and purified, with immunoreactivity confirmed against sera from naturally infected pigs. The dot blot test evaluated against serological gold-standard enzyme-linked immunotransfer blot (EITB) assay, demonstrated preliminary laboratory sensitivity (89 %, 95 % CI: 68.6-97.1) and specificity (100 %, 95 % CI: 97.6 - 100), highlighting its potential as a reliable alternative diagnostic method. Cost-effectiveness and minimal laboratory requirements make it particularly suited for field use in resource-limited settings.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the research.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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