Gold leaf electrodes for UV/Vis spectroelectrochemical determination of ortho-diphenols in extra virgin olive oil.

Autor: Abate M; Sustainable Analytical Instrumentation Laboratory, Department of Agrifood, Environmental and Animal Science, University of Udine, via Cotonificio 108, I-33100, Udine, Italy., Bontempelli G; Sustainable Analytical Instrumentation Laboratory, Department of Agrifood, Environmental and Animal Science, University of Udine, via Cotonificio 108, I-33100, Udine, Italy., Dossi N; Sustainable Analytical Instrumentation Laboratory, Department of Agrifood, Environmental and Animal Science, University of Udine, via Cotonificio 108, I-33100, Udine, Italy. Electronic address: nicolo.dossi@uniud.it.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2025 Mar 01; Vol. 284, pp. 127215. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1016/j.talanta.2024.127215
Abstrakt: A simple and easily replicable technique is here presented for assembling planar electrochemical cells based on gold-leaf electrodes using ordinary tools and a do-it-yourself approach. These devices have been profitably used to perform spectroelectrochemical measurements in the reflectance mode demonstrating for the first time how the use of gold-leaf electrodes can offer a viable and low cost alternative to traditionally used electrochemical platforms assembled by gold vapor deposition or screen printing. After optimization of fabrication parameters, the electrochemical and spectroelectrochemical characterization of these cells was performed by using potassium hexacyanoferrate(II) as redox probe. Then, hydroxytyrosol has been selected as model to develop an innovative and so far never presented spectroelectrochemical method for the determination and quantification of ortho-diphenol antioxidants in extra virgin olive oil. The results achieved allowed us to preliminary evaluate the electrochemical oxidation mechanism of hydroxytyrosol at gold leaf electrodes and then to select the best wavelength for its spectroelectrochemical determination. The approach developed enabled to evaluate hydroxytyrosol concentrations from both the electrochemical and spectroscopic signals with repeatable and comparable results. Comparison between electrochemical and optical determinations performed on real sample demonstrates that the two methods can be used indistinctively, proving that spectroelectrochemistry represents an autovalidated technique able to provide reliable results thanks to complementary information that are simultaneously obtained from electrochemistry and spectroscopy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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