| Autor: |
Xu J; School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China.; Center for Lipid Engineering, Muyuan Laboratory, 110 Shangding Road, Zhengzhou, Henan 450016, China., Dong H; School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China.; Center for Lipid Engineering, Muyuan Laboratory, 110 Shangding Road, Zhengzhou, Henan 450016, China., Chen S; School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China., Chang J; School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China.; Center for Lipid Engineering, Muyuan Laboratory, 110 Shangding Road, Zhengzhou, Henan 450016, China., Zhang W; Bloomage Biotechnology Corporation Limited, 678 Tianchen Street, Jinan, Shandong 250101, China., Zhao A; School of Life Sciences, Zhengzhou University, No.100 Science Avenue, Zhengzhou, Henan 450001, China., Alam MA; School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China., Wang S; School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China., Wang W; Department of Chemical Engineering, Stanford University, 443 Via Ortega, Shriram Center, Palo Alto, Stanford, California 94305, United States., Zhang J; NEW TUOYANG Bio-engineering Co., Ltd., No. 9 MoPing Road, Hebi, Henan 458000, China., Lv Y; School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China.; Center for Lipid Engineering, Muyuan Laboratory, 110 Shangding Road, Zhengzhou, Henan 450016, China.; NEW TUOYANG Bio-engineering Co., Ltd., No. 9 MoPing Road, Hebi, Henan 458000, China., Xu P; Center for Lipid Engineering, Muyuan Laboratory, 110 Shangding Road, Zhengzhou, Henan 450016, China.; Department of Chemical Engineering, Guangdong Technion-Israel Institute of Technology (GTIIT), 241 Daxue Road, Shantou, Guangdong 515063, China. |
| Abstrakt: |
D-Ribose plays fundamental roles in all living organisms and has been applied in food, cosmetics, health care, and pharmaceutical sectors. At present, D-ribose is predominantly produced by microbial fermentation based on the pentose phosphate pathway (PPP). However, this method suffers from a long synthetic pathway, severe growth defect of the host cell, and carbon catabolite repression (CCR). According to the Izumoring strategy, D-ribose can be produced from D-xylose through only three steps. Being not involved in the growth defect or CCR, this shortcut route is promising to produce D-ribose efficiently. However, this route has never been demonstrated in engineering practice, which hinders its application. In this study, we stepwise demonstrated this route and screened out higher active enzymes for each step. The first D-ribose production from D-xylose through the Izumoring route was achieved. By stepwise enzyme dosage tuning and process optimization, 6.87 g/L D-ribose was produced from 40 g/L D-xylose. Feeding D-xylose further improved the D-ribose titer to 9.55 g/L. Finally, we tested the coproduction of D-ribose and D-allose from corn stalk hydrolysate using the route engineered herein. In conclusion, this study demonstrated a pentose Izumoring route, complemented the engineering practices of the Izumoring strategy, paved the way to produce D-ribose from D-xylose, and provided an approach to comprehensively utilize the lignocellulosic sugars. |
