Seed priming with cold plasma, iron, and manganese nanoparticles modulates salinity stress in hemp (Cannabis sativa L.) by improving germination, growth, and biochemical attributes.

Autor: Ghasempour S; Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran., Ghanbari Jahromi M; Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran. ghanbari@iau.ir., Mousavi A; Department of Plant Molecular Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran., Iranbakhsh A; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2024 Dec; Vol. 31 (57), pp. 65315-65327. Date of Electronic Publication: 2024 Nov 23.
DOI: 10.1007/s11356-024-35590-y
Abstrakt: Using cutting-edge technologies such as non-thermal plasma and metallic nanoparticles has shown promise in ameliorating salinity-induced stress in plants. However, there are still knowledge gaps concerning the most effective strategies for mitigating salinity stress in hemp (Cannabis sativa L.) plants. Consequently, this study was undertaken to investigate the impact of seed priming with cold plasma (CP), iron (Fe), and manganese (Mn) nanoparticles (NPs) on salinity modulation in hemp plants, aiming to improve seed germination, plant growth, and biochemical attributes. A pot experiment was conducted with salinity at three levels (0, 60, and 120 mM NaCl) and seed priming at five levels (control, 60 s CP, 90 s CP, 50 mg L -1 Fe NPs, and 50 mg L -1 Mn NPs). The results showed that salinity at 120 mM decreased germination percentage (GP, 34%), germination speed (GS, 61%), seedling vigor index (SVI, 47%), shoot weight (38%), root weight (15%), chlorophyll (Chl) a + b (31%), and relative water content (RWC, 24%) while increasing malondialdehyde (MDA, 48%) and proline (39%). Seed priming with CP, Fe NPs, and Mn NPs increased plant weight, GP, GS, SVI, RWC, and Chl but decreased MDA accumulation. Fe NPs and salinity at 60 mM led to high levels of total phenolic content and total flavonoid content, which represent 29 and 44% increases, respectively, compared to the control. The heat map showed that among the treatments, GS and SVI had the highest degrees of variability. Fe NPs at 50 mg L -1 and CP at 90 s produced the best plant tolerance to salt stress.
Competing Interests: Declarations. Ethical approval: Ethical approval was not required for this work. Consent to participate: The author agreed to submit the manuscript to Environmental Science and Pollution Research. Consent for publication: The author approved the final manuscript to publish in Environmental Science and Pollution Research. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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