bHLH19 and bHLH20 repress jasmonate-mediated plant defense against insect herbivores in Arabidopsis.

Autor: Pang S; College of Life Sciences, Capital Normal University, Beijing, 100048, China., Zhai J; College of Life Sciences, Capital Normal University, Beijing, 100048, China., Song J; College of Life Sciences, Capital Normal University, Beijing, 100048, China., Rong D; College of Life Sciences, Capital Normal University, Beijing, 100048, China., Hong Y; Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China., Qiu Y; College of Life Sciences, Capital Normal University, Beijing, 100048, China., Ma J; College of Life Sciences, Capital Normal University, Beijing, 100048, China., Qi T; School of Life Sciences, Tsinghua University, Beijing, 100084, China., Huang H; Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China., Song S; College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Dec; Vol. 120 (6), pp. 2623-2638. Date of Electronic Publication: 2024 Nov 23.
DOI: 10.1111/tpj.17132
Abstrakt: Plants are attacked by various insect herbivores. Upon attack-triggered biosynthesis of the phytohormone jasmonates (JAs), the JA receptor CORONATINE INSENSITIVE 1 recruits the JA-ZIM domain (JAZ) repressors for ubiquitination, releases the MYC-MYB transcription factor (TF) complexes, and enhances glucosinolates (GSs) biosynthesis to promote defense against insects in Arabidopsis. However, the negative regulation of JA-regulated defense remains largely unclear. Here, we found that Arabidopsis IVa bHLH TFs bHLH19 and bHLH20 interacted with JAZs. The bhlh19/20 mutations enhanced defense against the insects Spodoptera frugiperda and S. exigua, while their overexpression inhibited defense. bHLH19/20 repressed defense via at least two layers of regulation: first, bHLH19/20 interacted with the members MYC2/3/4/5 and MYB34/51/122 of MYC-MYB complexes, and inhibited the interaction/transcription activity of MYC2-MYB34; second, bHLH19/20 activated the RNA level of nitrile-specifier protein 1, which converts GSs into the less toxic nitriles. bhlh19/20 exhibited no penalty in JA-regulated growth inhibition. Collectively, our findings reveal the molecular mechanism for negatively regulating JA-mediated defense against insects in Arabidopsis without growth penalty by the pair of bHLH19/20 TFs.
(© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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