Complete genome sequencing of Enterobacter ludwigii strain T977 revealed its great ability for starch degradation of Nicotiana tabacum L. Yunyan 97.

Autor: Hu L; Zhengzhou Tobacco Research Institute of CNTC, Henan Province, Zhengzhou, 450001, PR China. lwhures@outlook.com., Gao Q; Anhui Wannan Tobacco Co. Ltd, Xuancheng, An'Hui province, 242000, PR China. 2017792837@qq.com., Ji Y; Shandong Institute for Food and Drug Control, Jinan, Shandong Province, 250101, PR China., Dong X; Anhui Wannan Tobacco Co. Ltd, Xuancheng, An'Hui province, 242000, PR China., Zhu Q; Anhui Wannan Tobacco Co. Ltd, Xuancheng, An'Hui province, 242000, PR China., Cheng T; Anhui Wannan Tobacco Co. Ltd, Xuancheng, An'Hui province, 242000, PR China., Zhao L; Anhui Wannan Tobacco Co. Ltd, Xuancheng, An'Hui province, 242000, PR China., Yang M; Zhengzhou Tobacco Research Institute of CNTC, Henan Province, Zhengzhou, 450001, PR China., Zhen Zhai; Zhengzhou Tobacco Research Institute of CNTC, Henan Province, Zhengzhou, 450001, PR China., Dai H; Zhengzhou Tobacco Research Institute of CNTC, Henan Province, Zhengzhou, 450001, PR China., Liang T; Zhengzhou Tobacco Research Institute of CNTC, Henan Province, Zhengzhou, 450001, PR China., Xue C; Zhengzhou Tobacco Research Institute of CNTC, Henan Province, Zhengzhou, 450001, PR China.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2024 Nov 22; Vol. 24 (1), pp. 491. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1186/s12866-024-03613-w
Abstrakt: Enterobacter ludwigii has been proven by numerous studies to be an effective plant growth promoter. Enterobacter ludwigii T977 was isolated from leaves of Nicotiana tabacum L. Yunyan 97 which showing high starch degrading ability. The optimal fermentation carbon source of strain T977 was starch, with optimal starch concentration as 2.5 g/L, and the most suitable fermentation nitrogen source for the strain T977 was ammonium acetate, with optimal concentration as 0.25 g/L. The spaying treatment of strain T977 could reduce the starch content of upper leaves from 3.77% to 1.43%, the total sugar and reducing sugar decreased slightly, the starch content of middle leaves decreased from 5.63% to 3.18%, the content of total sugar and reducing sugar increased in middle leaves, and the other chemical components were in the appropriate range. Here, we reported 4.77 MB whole genome of a starch-degrading E. ludwigii T977 that encodes 4501 proteins, 11 α-amylases in GH13 family were identified, and the amylase (GM000159) with signal peptide may play important role in degradation of starch in tobacco leaves. Our study may provide an effective microbiological mean for reducing starch content in tobacco leaves.
Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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