Genome sequencing and comparative genomic analysis of bovine mastitis-associated non-aureus staphylococci and mammaliicocci (NASM) strains from India.

Autor: Ramesh V; Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021, India., Sivakumar R; Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021, India., Annamanedi M; National Institute of Animal Biotechnology, Hyderabad, 500032, India., Chandrapriya S; Department of Veterinary Microbiology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, 560024, India., Isloor S; Department of Veterinary Microbiology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, 560024, India., Rajendhran J; Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021, India. jrajendhran@gmail.com., Hegde NR; National Institute of Animal Biotechnology, Hyderabad, 500032, India. hegde@niab.org.in.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 22; Vol. 14 (1), pp. 29019. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1038/s41598-024-80533-9
Abstrakt: We describe the whole-genome sequencing and comparative genomic analysis of 22 mastitis-associated NASM strains isolated from India. The mean genome size of the strains was 2.55 Mbp, with an average GC content of 32.2%. We identified 14 different sequence types (STs) among the 22 NASM strains. Of these, ST1 and ST6 of S. chromogenes were exclusively associated with bovine mastitis. Genome-wide SNP-based minimum spanning tree revealed the intricate phylogenetic relationships among NASM strains from India, categorizing them into five major clades. Interestingly, mastitis-associated strains formed separate subclades in all the NASM species studied, indicating distinct host-specific co-evolution. The study identified 32 antimicrobial resistance (AMR) genes and 53 virulence-associated genes, providing insights into the genetic factors that could contribute to the pathogenicity of NASM species. Some virulence and AMR genes were found in the predicted genomic islands, suggesting possible horizontal transfer events.
Competing Interests: Declarations. Competing interests: We declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024. The Author(s).)
Databáze: MEDLINE
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