Walnut-derived peptides cross the blood-brain barrier and ameliorate Aβ-induced hypersynchronous neural network activity.

Autor: Min W; State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, PR China; College of Food and Health, Zhejiang A&F University, Hangzhou, Zhejiang 311300, PR China., Dang Q; College of Food Science and Engineering, Jilin Agricultural University, ChangChun, Jilin 130118, PR China., Li Z; College of Food Science and Engineering, Jilin Agricultural University, ChangChun, Jilin 130118, PR China; College of Food and Health, Zhejiang A&F University, Hangzhou, Zhejiang 311300, PR China., Zhao X; College of Food Science and Engineering, Jilin Agricultural University, ChangChun, Jilin 130118, PR China., Shen Y; College of Food Science and Engineering, Jilin Agricultural University, ChangChun, Jilin 130118, PR China., Fang L; College of Food Science and Engineering, Jilin Agricultural University, ChangChun, Jilin 130118, PR China., Liu C; College of Food Science and Engineering, Jilin Agricultural University, ChangChun, Jilin 130118, PR China. Electronic address: liuchunlei0709@jlau.edu.cn.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2024 Dec; Vol. 197 (Pt 2), pp. 115302. Date of Electronic Publication: 2024 Nov 06.
DOI: 10.1016/j.foodres.2024.115302
Abstrakt: Walnut peptides exhibit promising neuroprotective effects; however, they must be absorbed in their intact form through the gastrointestinal tract into the bloodstream and brain. In this study, the effects of the walnut peptide TWLPLPR (TW-7) were evaluated in mice, including its absorption and distribution ability to cross the blood-brain barrier, and inhibitory effects on hyperactivity of primary hippocampal neurons. TW-7 was stable in plasma, and the peptide retention rate was 88.19 ± 0.70 % after 48 h. In vitro imaging indicated that TW-7 was distributed in the brain, liver, lungs, and kidneys of mice after gavage, and an immunofluorescence analysis indicated that TW-7 could accumulate in mouse brain parenchyma; in addition, TW-7 reached its maximum concentration (5.36 ± 1.59 µg/mL) in plasma 2 h after gavage, and reached its peak concentration (0.95 ± 0.19 µg/g) in brain tissue 4 h after gavage. Microelectrode array and immunofluorescence analyses confirmed that TW-7 ameliorates the overexcitation of primary hippocampal neurons induced by Aβ 25-35 through inhibiting the excessive release of glutamate and protecting synaptic structure and function. These results suggest that TW-7 can penetrate the blood-brain barrier in mice and positively affect the electrophysiological activity of neurons. More broadly, these findings provide a theoretical basis for the development and application of walnut peptide-based functional food for Alzheimer's disease intervention.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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