Vibrotactile stimulation at 40Hz inhibits Aβ-induced changes in SH-SY5Y, BV2 cells, and pericytes.

Autor: Shin CH; Department of AI Convergence Biomedical Engineering, Dongguk University, Goyang-si, 10326, Republic of Korea; AriBio Co., Ltd., 56, Dongpangyo-ro, Bundang-gu, Seongnam-si, 13535, Republic of Korea., Kang BW; AriBio Co., Ltd., 56, Dongpangyo-ro, Bundang-gu, Seongnam-si, 13535, Republic of Korea., Cho MW; AriBio Co., Ltd., 56, Dongpangyo-ro, Bundang-gu, Seongnam-si, 13535, Republic of Korea., Ha JY; AriBio Co., Ltd., 56, Dongpangyo-ro, Bundang-gu, Seongnam-si, 13535, Republic of Korea., Choung JJ; AriBio Co., Ltd., 56, Dongpangyo-ro, Bundang-gu, Seongnam-si, 13535, Republic of Korea., Song DK; AriBio Co., Ltd., 56, Dongpangyo-ro, Bundang-gu, Seongnam-si, 13535, Republic of Korea., Ko HK; School of Psychology & Clinical Language Sciences, University of Reading, Reading, RG6 6AH, United Kingdom., Nam MH; Department of Medical Biotechnology, Dongguk University, Goyang-si, 10326, Republic of Korea., Seo YK; Department of Medical Biotechnology, Dongguk University, Goyang-si, 10326, Republic of Korea. Electronic address: bioseo@dongguk.edu.
Jazyk: angličtina
Zdroj: Brain research bulletin [Brain Res Bull] 2024 Nov 20, pp. 111138. Date of Electronic Publication: 2024 Nov 20.
DOI: 10.1016/j.brainresbull.2024.111138
Abstrakt: Alzheimer's disease (AD) poses a major societal challenge, yet no definitive cure exists. Noninvasive brain stimulation methods, such as transcranial magnetic stimulation and transcranial direct current stimulation, have shown promise in alleviating cognitive symptoms associated with neurodegenerative disorders. This study investigated the effects of 40Hz vibrotactile stimulation on AD-related cellular responses using SH-SY5Y neuroblastoma cells, primary human brain pericytes, and BV2 microglia. SH-SY5Y cells and brain pericytes treated with oligomeric beta-amyloid (Aβ) underwent 40Hz vibrational stimulation for varying durations. Cell viability was determined via the CCK-8 assay, while intracellular calcium levels in pericytes were assessed. Protein expression was measured using western blotting, and gene expression was quantified via a real-time quantitative polymerase chain reaction. Detailed vibrational parameters were employed to ensure precise stimulation. Notably, 40Hz vibrotactile stimulation improved cell viability in Aβ-exposed SH-SY5Y cells, reduced intracellular calcium ion (Ca2+) levels in Aβ-treated pericytes, activated autophagy, and mitigated tau hyperphosphorylation in SH-SY5Y cells. Additionally, it exhibited anti-neuroinflammatory properties in BV2 microglia. These findings highlight the potential of 40Hz vibrotactile stimulation as a therapeutic strategy for AD.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
Externí odkaz: