Tetrahedral DNA nanostructures, graphene and carbon nanodots-based electrochemiluminescent biosensor for BRCA1 gene mutation detection.

Autor: García-Fernández D; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049, Madrid, Spain., Gutiérrez-Gálvez L; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049, Madrid, Spain., López-Diego D; Instituto de Micro y Nanotecnología IMN-CNM. CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain., Luna M; Instituto de Micro y Nanotecnología IMN-CNM. CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain., Torres Í; Departamento de Química Inorgánica and Condensed Matter Physics Center (IFIMAC), Universidad Autónoma de Madrid. 28049, Madrid Spain., Zamora F; Departamento de Química Inorgánica and Condensed Matter Physics Center (IFIMAC), Universidad Autónoma de Madrid. 28049, Madrid Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain., Solera J; Molecular Oncogenetics Unit, La Paz Universitary Hospital, Biochemistry Department, Faculty of Medicine, Universidad Autónoma de Madrid, Madrid 28046, Spain., García-Mendiola T; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049, Madrid, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain. Electronic address: tania.garcia@uam.es., Lorenzo E; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049, Madrid, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2025 Mar 01; Vol. 284, pp. 127182. Date of Electronic Publication: 2024 Nov 10.
DOI: 10.1016/j.talanta.2024.127182
Abstrakt: In this study, we present a novel electrochemiluminescent DNA biosensor designed for detecting breast cancer type 1 (BRCA1) gene mutations. The biosensor integrates graphene nanosheets (Graph-NS), tetrahedral DNA nanostructures (TDNs), and carbon nanodots (CNDs) to enhance sensitivity and specificity. Graph-NS are employed to structure the transducer and serve as a platform for DNA immobilization. TDNs are engineered with a BRCA1 gene-specific capture probe located at the apex (TDN-BRCA1), facilitating efficient biorecognition. Additionally, the basal vertices of TDNs are functionalized with amino groups, enabling their attachment to the CSPE/Graph-NS surface via amino-graphene interaction. This platform effectively identifies single-base mutations in the BRCA1 gene utilizing synthesized CNDs as a coreactant and [Ru(bpy) 3 ] 2+ as the luminophore through the coreactant pathway. The developed biosensor demonstrates exceptional sensitivity and can detect a single mutation in the BRCA1 gene. Furthermore, it has been successfully validated in real samples obtained from breast cancer patients, showcasing a remarkable detection limit of 1.41 aM.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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