Protocol for evaluating extracellular matrix stiffness post-decellularization of triple-negative breast cancer cells using atomic force microscopy.
| Autor: | Adhikari S; Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India; Homi Bhabha National Institute, Mumbai, India., Ghosal M; Chemical Sciences Division, Saha Institute of Nuclear Physics, HBNI, 1/AF Bidhannagar, Kolkata 700064, India; Homi Bhabha National Institute, Mumbai, India., Kabiraj A; Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India; Homi Bhabha National Institute, Mumbai, India., Senapati D; Chemical Sciences Division, Saha Institute of Nuclear Physics, HBNI, 1/AF Bidhannagar, Kolkata 700064, India; Homi Bhabha National Institute, Mumbai, India. Electronic address: dulal.senapati@saha.ac., Das C; Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India; Homi Bhabha National Institute, Mumbai, India. Electronic address: chandrima.das@saha.ac.in. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Dec 20; Vol. 5 (4), pp. 103460. Date of Electronic Publication: 2024 Nov 21. |
| DOI: | 10.1016/j.xpro.2024.103460 |
| Abstrakt: | Atomic force microscopy (AFM) is a versatile and widely used technique in the field of current biomedical research. Here, we present a protocol for measuring the stiffness property of extracellular matrix (ECM) gel, enriched by breast cancer cells. We describe steps for applying the PeakForce quantitative nanomechanics technique after scoring the elastic modulus and capturing topology images. This protocol has implications for determining the contribution of cancer cells in modulating the stiffness of artificial ECM gel. For complete details on the use and execution of this protocol, please refer to Adhikari et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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