Detection and assessment of antimicrobial resistance promotion risk in the Little Akaki River wastewater.

Autor: Gessew GT; Department of Medical Laboratory Science, College of Health Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia. gebreab4@gmail.com., Desta AF; Department of Microbial, Cellular, and Molecular Biology, College of Natural Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia.
Jazyk: angličtina
Zdroj: Environmental monitoring and assessment [Environ Monit Assess] 2024 Nov 22; Vol. 196 (12), pp. 1238. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1007/s10661-024-13448-7
Abstrakt: The misuse of antimicrobial agents in healthcare and animal farming has led to their release into the environment through wastewater, contributing to the emergence of resistant bacteria. This study analyzed selected antimicrobial concentration and antimicrobial resistance (AMR) risk in the Little Akaki River wastewater. Samples collected from March to December 2019, June 2020, and August 2022 were processed using solid phase extraction with a hydrophilic-lipophilic balance (HLB) cartridge and analyzed through ultra-high-performance liquid chromatography (UHPLC) with mass spectrometry. Risk quotients (RQ) were calculated as the ratio of measured environmental concentrations (MEC) to predicted no-effect concentrations (PNEC). Ciprofloxacin concentrations ranged from 5.62 to 9.34 µg/L, cefotaxime from 1.89 to 64.79 µg/L, and sulfamethoxazole from 29.11 to 248.77 µg/L, with higher concentrations observed during the wet season. MECs for ciprofloxacin, cefotaxime, and sulfamethoxazole exceeded their PNECs (RQ > 2), indicating a high risk of resistance development. Sulfamethoxazole posed the greatest risk due to its consistently elevated levels. In contrast, erythromycin concentrations remained below the PNEC, suggesting minimal resistance risk. These findings highlight the urgent need for wastewater treatment strategies to mitigate AMR risks in aquatic environments.
Competing Interests: Declarations. Ethics approval and consent to participate: All authors have read, understood, and have complied as applicable with the statement on “Ethical responsibilities of Authors” as found in the “Instructions for Authors”. Consent for publication: Not applicable Conflict of interest: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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