Transcriptional profiling reveals the role of Candida albicans Rap1 in oxidative stress response.
| Autor: | Wang WH; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 300044, Taiwan., Chen HY; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 300044, Taiwan., Chen SY; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 300044, Taiwan., Lan CY; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 300044, Taiwan.; Department of Life Science, National Tsing Hua University, Hsinchu 300044, Taiwan.; School of Medicine, National Tsing Hua University, Hsinchu 300044, Taiwan. |
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| Jazyk: | angličtina |
| Zdroj: | Bioscience reports [Biosci Rep] 2024 Dec 17; Vol. 44 (12). |
| DOI: | 10.1042/BSR20240689 |
| Abstrakt: | Candida albicans is a member of the human commensal microbiota but can also cause opportunistic infections, including life-threatening invasive candidiasis, particularly in immunocompromised patients. One of the important features of C. albicans commensalism and virulence is its ability to adapt to diverse environmental stress conditions within the host. Rap1 is a DNA-binding protein identified in yeasts, protozoa, and mammalian cells, and it plays multiple functions, including telomere regulation. Intriguingly, our previous study showed that Rap1 is also involved in cell wall integrity, biofilm formation, and virulence in C. albicans. In this work, using RNA-seq analysis and other approaches, the role of C. albicans Rap1 in oxidative stress response was further revealed. The RAP1-deletion mutant exhibited greater resistance to the superoxide generator menadione, a lower level of intracellular reactive oxygen species (ROS) upon menadione treatment, and higher expression levels of superoxide dismutase genes, all in response to oxidative stress. Moreover, the association between Rap1-mediated oxidative stress response and the mitogen-activated protein kinase (MAPK) Hog1, the transcription factor Cap1 and the TOR signalling was also determined. Together, these findings expand our understanding of the complex signalling and transcriptional mechanisms regulating stress responses in C. albicans. (© 2024 The Author(s).) |
| Databáze: | MEDLINE |
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