Mitomycin C eliminates cyanobacterial transcription without detectable lysogen induction in a Microcystis -dominated bloom in Lake Erie.
| Autor: | Martin RM; Department of Microbiology, University of Tennessee at Knoxville, Knoxville, TN, USA., Denison ER; Department of Microbiology, University of Tennessee at Knoxville, Knoxville, TN, USA., Pound HL; Department of Microbiology, University of Tennessee at Knoxville, Knoxville, TN, USA., Barnes EA; Department of Microbiology, University of Tennessee at Knoxville, Knoxville, TN, USA., Chaffin JD; F.T. Stone Laboratory, Ohio Sea Grant, and The Ohio State University, Put-In-Bay, OH, USA., Wilhelm SW; Department of Microbiology, University of Tennessee at Knoxville, Knoxville, TN, USA. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Nov 07. Date of Electronic Publication: 2024 Nov 07. |
| DOI: | 10.1101/2024.11.06.622312 |
| Abstrakt: | Although evidence indicates that viruses are important in the ecology of Microcystis spp., many questions remain. For example, how does Microcystis exist at high, bloom-associated cell concentrations in the presence of viruses that infect it? The phenomenon of lysogeny and associated homoimmunity offer possible explanations to this question. Virtually nothing is known about lysogeny in Microcystis , but a metatranscriptomic study suggests that widespread, transient lysogeny is active during blooms. These observations lead us to posit that lysogeny is important in modulating Microcystis blooms. Using a classic mitomycin C-based induction study, we tested for lysogeny in a Microcystis -dominated community in Lake Erie in 2019. Treated communities were incubated with 1 mg L -1 mitomycin C for 48 h alongside unamended controls. We compared direct counts of virus-like-particles (VLPs) and examined community transcription for active infection by cyanophage. Mitomycin C treatment did not increase VLP count. Mitomycin C effectively eliminated transcription in the cyanobacterial community, while we detected no evidence of induction. Metatranscriptomic analysis demonstrated that the standard protocol of 1 mg L -1 was highly-toxic to the cyanobacterial population, which likely inhibited induction of any prophage present. Follow-up lab studies indicated that 0.1 mg L -1 may be more appropriate for use in freshwater cyanobacterial studies. These findings will guide future efforts to detect lysogeny in Microcystis blooms. Competing Interests: Conflict of Interest: The authors declare no conflicts of interest. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|