Molecular and functional adaption of Arabidopsis villins.

Autor: Li X; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China., Zhuang Y; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China., Zhao W; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China., Qu X; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.; Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, China., Wang J; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China., Chang M; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China., Shen J; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China., Chen N; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China., Huang S; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Nov 22. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1111/nph.20295
Abstrakt: Villins are versatile, multifunctional actin regulatory proteins. They promote actin stabilization and remodeling mainly via their actin bundling and Ca 2+ -dependent severing activities, respectively. Arabidopsis subclass II and III villins normally coexist in cells, but the biological significance of their coexistence remains unknown. Here we demonstrate that subclass II villin binds to Ca 2+ with high affinity and exhibits strong severing but weak bundling activity compared to subclass III villin. Subclass II villin plays a dominant role in promoting actin remodeling, which requires its Ca 2+ -dependent severing activity. Subclass II villin is also strictly required for physiological processes including oriented organ growth and stress tolerance. By comparison, subclass III villin binds to Ca 2+ with low affinity and exhibits weak severing but strong bundling activity, and acts as the major player in controlling actin stabilization and organization. Thus, we demonstrate that multifunctional villin isovariants have diverged biochemically to ensure exquisite control of the actin cytoskeleton to meet different cellular needs in plants. This study provides new insights into the role of villins in fine-tuning actin dynamics and plant development.
(© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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