Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.

Autor: Ninomiya Y; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan., Matsuda S; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. matsudas@hiroshima-u.ac.jp., Suzuki S; Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan.; Department of Operative Dentistry, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Sendai, Japan., Hirata-Tsuchiya S; Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan., Ueda T; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan., Nakashima F; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan., Yasuda K; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan., Shimada S; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan., Memida T; Department of Oral Science and Translation Research, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, USA., Yoshimoto T; Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan., Yamada S; Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan., Ouhara K; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan., Mizuno N; Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
Jazyk: angličtina
Zdroj: BMC oral health [BMC Oral Health] 2024 Nov 21; Vol. 24 (1), pp. 1422. Date of Electronic Publication: 2024 Nov 21.
DOI: 10.1186/s12903-024-05211-8
Abstrakt: Background: This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).
Methods: Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.
Results: RNA sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.
Conclusion: BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.
Competing Interests: Declarations. Ethics approval and consent to participate: The study protocol was approved by the research ethics committees of Hiroshima University (E2015-0001), and written informed consent was obtained from the subjects. Consent for publication: Consent for publication was obtained from the participants. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: